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Molecular marker related to growth and development characters of broilers and application thereof

A molecular marker, growth and development technology, applied in the field of poultry genetics and breeding, can solve problems such as unclear etiology and achieve the effect of improving production performance

Active Publication Date: 2019-09-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the cause of RSS chickens is still unclear, there is currently no effective method available to control the disease

Method used

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  • Molecular marker related to growth and development characters of broilers and application thereof
  • Molecular marker related to growth and development characters of broilers and application thereof
  • Molecular marker related to growth and development characters of broilers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for eliminating "stiff chickens" by using molecular markers related to growth and development traits of broiler chickens, comprising the steps of:

[0027] 1. Collection of chicken blood samples and DNA extraction:

[0028] After the determination of the growth traits of the tested chickens is completed, about 1mL of blood is drawn from the vein under the chicken wing with a disposable syringe, and injected with about 200 μL of 2% sterile EDTA (Ethylene diamine tetraacetic acid, EDTA) anticoagulant after autoclaving. In a 1.5mL centrifuge tube, shake gently, record the wing number, and store at -80°C for later use.

[0029] Genomic DNA was extracted using the phenol-chloroform extraction method:

[0030] (1) Put 30 μL of whole blood into a 1.5 mL centrifuge tube, add 470 μL of 1×SET buffer, 12.5 μL of 20% SDS and 6 μL of 10 mg / mL proteinase K, mix well and place in a 55°C water bath overnight;

[0031] (2) Take out the sample in a 1.5mL centrifuge tube, add 5...

Embodiment 2

[0048] The invention is a detection application for correlation analysis between different genotypes of TWNK gene polymorphic sites and growth and development traits of chickens.

[0049] Result is as shown in table 2, can draw 2 kinds of genotypes to chicken's daily gain (p<0.05), carcass weight (p<0.01), leg muscle weight (p<0.01), breast muscle weight (p<0.01) by table 2. p<0.05) and abdominal fat weight (p<0.05) reached a significant or extremely significant level. The growth and development traits of GG genotype individuals were significantly higher than those of AA genotype individuals, which was the dominant genotype.

[0050] Table 2. Association analysis between different genotypes of TWNK c.409G>A and growth and development traits

[0051]

[0052] Therefore, TWNK gene marker-assisted selection can be used to eliminate heterozygous chickens in broiler breeding, avoid the generation of "dead chickens", and solve the problem of continuous occurrence of dead chicken...

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Abstract

The invention discloses a molecular marker related to growth and development characters of broilers and application thereof. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:1, and the mutation of the molecular marker is located at exon 1c.409G>A (p.Ala137Thr). The invention discovers that the growth of the broilers is further blocked due to the mitochondrial DNA depletion caused by the TWNK gene mutation, and the production character of the broilers is influenced. Therefore, the molecular marker can be applied to eliminating stiff chickens, and the application comprises the following steps of: S1, extracting DNA of a chicken to be detected; S2, acquiring and sequencing a target fragment: amplifying DNA of the chicken to be detected through PCR by using a forward and reverse primer pair, sequencing the obtained product, and analyzing sequence bases; and S3, according to the analysis result, selecting individuals which are determined to retain the GG genotype during breeding. The molecular marker is favorable for breeding dominant homozygous genotype broilers and improveing the production performance of the broilers.

Description

technical field [0001] The invention relates to the technical field of poultry genetics and breeding, in particular to a molecular marker related to growth and development traits of broiler chickens and an application thereof. Background technique [0002] my country has abundant high-quality broiler chicken resources, and growth is a very important economic trait in livestock and poultry production. However, even for the same breed of broiler chickens, there are obvious differences in the growth and development of different individuals. Therefore, the growth and development traits of broilers have always been the key research object of poultry breeding, and the study of growth and development traits is of great significance to the growth and breeding of broilers. [0003] With the rapid development of modern molecular biology technology, the new technology can quickly and accurately analyze the genetic composition of livestock and poultry from the molecular level, so as to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张细权胡博文李红梅张德祥聂庆华罗庆斌罗文胡霜杨敏敏廖志赢
Owner SOUTH CHINA AGRI UNIV
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