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Molecular marker closely related to rape flower color traits and application of molecular marker

A molecular marker and flower color technology, applied in the fields of rapeseed breeding and molecular biology, to achieve the effects of enhancing purpose, improving selection efficiency, and simple detection methods

Active Publication Date: 2019-03-29
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far no functional markers have been applied to the selection of Brassica napus

Method used

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  • Molecular marker closely related to rape flower color traits and application of molecular marker
  • Molecular marker closely related to rape flower color traits and application of molecular marker
  • Molecular marker closely related to rape flower color traits and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A molecular marker LJ04 closely related to the white flower trait of rapeseed was obtained by the following method:

[0016] In this example, the DH segregation population developed from one part of white-flowered rapeseed and one part of yellow-flowered rapeseed is taken as an example, and the method for obtaining molecular markers closely related to the white-flowered trait of rapeseed is described in detail, as follows:

[0017] 1. Population construction and phenotypic identification

[0018] The F1 generation was produced by crossing a part of white rapeseed W1 with a part of yellow rapeseed Y1, and then the F1 generation was developed into a DH population through microspore culture technology. During the full flowering period of the plant, the petals of the newly bloomed flowers on the day were scanned, and the B value in RGB was extracted as the phenotype data.

[0019] 2. SNP microarray analysis

[0020] For 91 DH lines, the DNA of young leaves was extracted a...

Embodiment 2

[0034] The difference between this example and Example 1 is that the genotypes of the rapeseed parents in this example are different, and the others are the same as those in Item 4 of Example 1, "Transformation and Detection of Functional Markers".

[0035] In this example, 101 DH lines developed from white-flowered rapeseed W2 and yellow-flowered rapeseed Y2 were used as research materials. Among these plants, 56 showed white flowers and 45 showed yellow flowers. Using the marker LJ04 developed in Example 1 for identification and detection of PCR amplification products: after separation by 13% polyacrylamide gel electrophoresis, only 57 materials with a single band of 177bp appeared, which were white flower parent W2 and 56 materials White flower DH line; only 180bp specific band appeared in 46 materials, which belonged to the yellow flower parent Y2 and 45 yellow flower DH lines; only one material showed both 177bp and 180bp bands, which was F1, and the flower color was white...

Embodiment 3

[0037] The difference between the present embodiment and the embodiment 2 is that the isolated populations of rape in the present embodiment are different, and the others are the same as the embodiment 2.

[0038] In this example, the F1 self-crossing in Example 2 was used to generate the F2 generation, and 255 F2 individuals were tested according to the same detection method in Example 2. The results are as follows: only 177bp single band appeared in 66 materials, all of which appeared as white flowers; only 180bp specific band appeared in 59 materials, all of which appeared as yellow flowers; 120 materials showed both 177bp and 180bp bands , for F1, the flower color is white (such as image 3 ).

[0039] Among the above-mentioned F2 materials, 5 materials (white flowers) with only a single band of 177bp, 5 materials with a specific band of 180bp (yellow flower), and 12 materials (white) with two bands of 177bp and 180bp at the same time were selected. F2: 3 families were p...

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Abstract

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker closely related to the rape flower color traits and application of the molecular marker. The molecular marker comprises a forward primer and a reverse primer. With total DNA as a template, PCR amplification is performed on the molecular marker and electrophoretic separation is performed; a single plant only having a single stripe of 180 bp is a homozygous yellow-flower individual, a single plant only having a single stripe of 177 bp is a homozygous white-flower individual, anda single plant having the two stripes at the same time is a heterozygous individual. The molecular marker developed by the invention can be applied to identification and prediction of a white-flower variety and a yellow-flower variety of rape; the operation is simple and convenient; a method is easy to implement; the problem of identification between a heterozygous genotype and a homozygous genotype can be effectively solved; offspring selection is more pertinent; the selection efficiency is improved.

Description

technical field [0001] The invention belongs to the fields of rapeseed breeding and molecular biology, and mainly relates to a molecular marker closely related to rapeseed flower color traits and its application. Background technique [0002] Flower color is one of the important traits of plants, which has the functions of attracting and directing pollinators, protecting floral organs, and maintaining the energy balance of floral tissues. The widely planted rapeseed is mainly yellow flowers, but at present, breeders have developed white, red, orange, pink, pink, pink and other colored rapeseed flowers, and the yield is similar to that of ordinary yellow rapeseed. In addition to ensuring the output of rapeseed, the colorful rapeseed flower also enhances the ornamental value, economic value and social value of rapeseed. Colored rapeseed flowers are selected through distant hybridization, recurrent selection, directional backcrossing, etc. At present, the cultivation of colore...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 梅家琴杨茹涵钱伟王金花
Owner SOUTHWEST UNIVERSITY
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