Molecular marker closely related to rape flower color traits and application of molecular marker

A molecular marker and flower color technology, applied in the fields of rapeseed breeding and molecular biology, to achieve the effects of enhancing purpose, improving selection efficiency, and simple detection methods
CN109536638AActive Publication Date: 2019-03-29SOUTHWEST UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHWEST UNIVERSITY
Publication Date
2019-03-29

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Abstract

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker closely related to the rape flower color traits and application of the molecular marker. The molecular marker comprises a forward primer and a reverse primer. With total DNA as a template, PCR amplification is performed on the molecular marker and electrophoretic separation is performed; a single plant only having a single stripe of 180 bp is a homozygous yellow-flower individual, a single plant only having a single stripe of 177 bp is a homozygous white-flower individual, anda single plant having the two stripes at the same time is a heterozygous individual. The molecular marker developed by the invention can be applied to identification and prediction of a white-flower variety and a yellow-flower variety of rape; the operation is simple and convenient; a method is easy to implement; the problem of identification between a heterozygous genotype and a homozygous genotype can be effectively solved; offspring selection is more pertinent; the selection efficiency is improved.
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Description

technical field

[0001] The invention belongs to the fields of rapeseed breeding and molecular biology, and mainly relates to a molecular marker closely related to rapeseed flower color traits and its application. Background technique

[0002] Flower color is one of the important traits of plants, which has the functions of attracting and directing pollinators, protecting floral organs, and maintaining the energy balance of floral tissues. The widely planted rapeseed is mainly yellow flowers, but at present, breeders have developed white, red, orange, pink, pink, pink and other colored rapeseed flowers, and the yield is similar to that of ordinary yellow rapeseed. In addition to ensuring the output of rapeseed, the colorful rapeseed flower also enhances the ornamental value, economic value and social value of rapeseed. Colored rapeseed flowers are selected through distant hybridization, recurrent selection, directional backcrossing, etc. At present, the cultivation of colore...

Claims

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