Method for detecting genotype of chicken beard

A genotype and beard technology, applied in the field of molecular biology, can solve the problems of long-term dominant trait genes, speed up seed selection, purification, etc., and achieve the effect of low cost, high accuracy and simple operation

Active Publication Date: 2015-02-11
CHINA AGRI UNIV
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Problems solved by technology

Traditional phenotyping methods take a long time to purify genes for dominant traits
At present, the molecular marker method for identifying the genotype of the beard trait has not been

Method used

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  • Method for detecting genotype of chicken beard
  • Method for detecting genotype of chicken beard
  • Method for detecting genotype of chicken beard

Examples

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Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Determination of polymorphic sites and establishment of pyrosequencing method

[0029] 1. Determination of polymorphic sites

[0030]In order to analyze the chicken beard genotype, the present invention provides the SNP site for detecting the beard genotype. The identification of the SNP site was carried out by the original and newly generated copies of three CNVs specific to chromosome 27 bearded chickens in 7 bearded individuals (HH) and 16 (species) wild-type individuals (hh) of the three breeds Sequencing analysis. A total of 2099 SNP sites were found in the sequencing results, of which only 1707859bp of this SNP site was genotype CC in the newly generated copies of CNV1 of all beard individuals, and TT in the original copies. The resequencing results of bearded chicken (HH) and Lingnan yellow chicken A03 line (hh) also supported this result. In bearded chicken (HH), this point is CT heterozygous and C:T=1:1, in A03 chicken ( hh) This point is TT. From...

Embodiment 2

[0047] Example 2 Application of Molecular Markers

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Abstract

The invention provides a method for detecting genotype of chicken beard and relates to the field of molecular biology. The method provided by the invention is based on a pyrosequencing technology, a pair of primers and a sequencing primer with the amino acid sequences as shown in SEQ ID No. 1, 2 and 3 are adopted to analyze single nucleotide polymorphism of a 1707859bp locus of a chicken No. 27 chromosome, and the results find that the genotype of the chicken beard is that, dominant homozygotes have T bases and C bases, which have the same peak height and account for one half respectively; in heterozygotes, the peak height of T bases is greater than that of the C bases, and T: C=2: 1; and recessive homozygotes only have peaks of the T bases and no peaks of the C bases. The method provided by the invention is simple to operate, high in sensitivity, strong in accuracy, high in throughput and low in detection cost, can fast detect beard individuals with the homozygous genotype and has importable application value in chicken conservation and breeding processes.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a SNP molecular marker used for detecting chicken beard genotypes and a method for detecting beard genotypes. Background technique [0002] Single nucleotide polymorphism markers (SNPs) mainly refer to DNA sequence polymorphisms caused by single nucleotide mutations on the genome. Detection of genotype by detection of single nucleotide polymorphism markers is a method that has emerged in recent years. Molecular markers have been used in animal breeding for some time. Compared with traditional breeding methods, molecular breeding greatly speeds up the efficiency of breeding, saves breeding time, and enables breeders to continuously explore and select better animals at the molecular level. Livestock breeds. [0003] A variety of methods have been used for SNP detection. Currently, gene chip methods, DNA sequencing methods, mass spectrometry methods, and TaqMan fluoresce...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 胡晓湘郭影顾晓荣盛哲雅舒鼎铭罗成龙李宁
Owner CHINA AGRI UNIV
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