A transgenic method using wheat anthers as receptors

A technology of transgenic plants and receptors, applied in the field of transgenic, can solve the problems of complex chromosome composition and slow stability of transgenic offspring, and achieve the effect of improving the efficiency of resource innovation
CN102277385AInactive Publication Date: 2011-12-14CROP RES INST SHANDONG ACAD OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
CROP RES INST SHANDONG ACAD OF AGRI SCI
Publication Date
2011-12-14
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a transgenic method using wheat anthers as receptors. The method provided by the invention comprises the following steps: 1) using plant anthers as receptors to carry out gene transformation with a gene gun method to obtain transformed anthers; 2) culturing the transformed anthers obtained in step 1) to obtain embryoid bodies, The embryoid bodies are then cultured to obtain haploid regenerated shoots. The present invention establishes a wheat transgenic technology system with anthers as receptors, in vitro moisturizing of anthers, adhesion and fixation of anthers during gene gun bombardment, gold powder particle size for preparing bullets, bullet coating effect, bombardment distance and haploid Chromosome doubling and other processes have been systematically studied, and transgenic plants with homozygous genotypes can be obtained in contemporary times.
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Description

technical field

[0001] The invention relates to a transgenic method using wheat anthers as receptors. Background technique

[0002] In 1991, Vasil et al introduced the bar gene into wheat by gene gun-mediated method, and obtained the world's first wheat transgenic plant. In the following year, Weeks et al. introduced the gus gene and bar gene into wheat using the gene gun-mediated method, and established a more mature technical system for gene gun transformation of young wheat embryos by optimizing different parameters in the process of gene gun transformation. Afterwards, wheat gene transformation has made significant progress. Different methods were used to introduce multiple genes into wheat immature embryos, and a batch of wheat transgenic plants were obtained, which provided data for the genetic research of exogenous genes. However, compared with rice, corn and other crops, wheat is the most difficult food crop for genetic transformation, and the research on wheat gene...

Claims

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