SNP marker associated with vibrio harveyi disease of large yellow croaker and primer and application thereof

Abstract

The invention relates to the technical field of molecular identification of fish, in particular to an SNP marker associated with vibrio harveyi disease of large yellow croaker and primers and applications thereof, wherein the nucleotide sequence of the SNP marker is shown as SEQ ID NO. 1, and the nucleotide sequence starts from base G or T at position 343 from 5 'end. The invention overcomes the problem that there is no SNP marker which can be used for the selection and breeding of disease resistance related traits of large yellow croaker in the prior art, and at the SNP site of the invention,the probability of the large yellow croaker with the genotype of GT to be infected with Vibrio harveyi is significantly higher than that of the homozygous GG genotype individual. Therefore, by detecting the SNP of Pseudosciaena crocea, it can be effectively determined whether it is susceptible to Vibrio harveyi infection. Elimination of GT genotype individual in parent breeding is favorable for improve that ability of offspring to resist infection of Vibrio harveyi, and the marker of the invention is used for auxiliary breed, thereby accelerating the cultivation process of disease-resistant varieties of large yellow croaker.

Description

technical field [0001] The invention relates to the technical field of fish molecular identification, in particular to a SNP marker associated with Vibrio harveii disease of large yellow croaker and its primer and application. Background technique [0002] Large yellow croaker (Larimichthys crocea) is one of the important economic fish in my country's coastal waters. With the rapid expansion of large yellow croaker artificial breeding scale and the continuous improvement of intensification, large yellow croaker breeding diseases caused by bacteria, parasites, viruses, etc. continue to appear and become more serious. Among them, vibriosis has a long onset time, high morbidity and mortality, and has become an important factor restricting the industrialization of large yellow croaker farming. Harvey vibriosis caused by Vibrio harveyi is the most common in large yellow croaker farming one of the most contagious vibrio diseases. In order to control Vibrio harveyi disease, farme...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem that there is no SNP marker that can be used for the breeding of large yellow croaker disease-resistant traits in the prior art, and provides a method that can be effectively used for the breeding of large yellow croaker disease-resistant traits, and quickly detects the resistance of large yellow croaker. Harvey vibriosis resistance of a large yellow croaker harvet vibriosis-associated SNP marker and its primers and applications

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