Method for detecting Marek's disease resistance homozygous genotype chicken

A technology of Marek's disease and homozygous genes, applied in the field of biology, can solve the problems of high breeding costs, tedious progeny determination, large number of basic groups, etc., and achieve considerable economic benefits

Inactive Publication Date: 2010-08-04
GUANGXI UNIV
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Problems solved by technology

These methods are the simplest, but the disadvantages are the large number of basic populations required, a special challenge environment, and tedious progeny determination, and the high cost of breeding

Method used

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  • Method for detecting Marek's disease resistance homozygous genotype chicken
  • Method for detecting Marek's disease resistance homozygous genotype chicken
  • Method for detecting Marek's disease resistance homozygous genotype chicken

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Experimental program
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Embodiment

[0038] 1. Results of chicken challenge test

[0039] According to the content of the invention, the chicken challenge test was carried out. After the challenge, the test ended in 13 weeks. MD resistant Xiayan chickens survived 14 feathers, and common Xiayan chickens survived 9 feathers (Table 1). The number of occurrences was far less than that of common Xiayan chickens, so individuals with significant differences in MD resistance and susceptibility were obtained.

[0040] Table 1. The presence or absence of tumors, death and survival in experimental chickens after challenge

[0041]

[0042] Note: Group K means MD-resistant chickens (groups C and D), and group Y means ordinary Xiayan chickens (groups A and B); the unit in the table is "feather".

[0043] Table 2 shows the tumor mortality and tumor incidence rates of chickens in groups A, B, C, and D. The t test shows that the tumor mortality rate (43.38%) of MD-resistant chickens is significantly lower (p<0.05) than that...

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Abstract

The invention discloses a method for detecting Marek's disease resistance homozygous genotype chicken, which comprises the following steps: adopting the PCR-RFLP technology to perform PCR amplification on the second exon of BLB2 genes of 99 MDV-1 toxin eliminated Xiayan chickens and perform Alu I, Cai I, Cfr I, Hinl I, Hinf I and Rsa I restriction enzyme analysis on the PCR products, and initially obtaining seven BLB2 allelic genes genotype chickens by performing the restriction analysis on the six loci; finally obtaining six BLB2/BF2 allelic genes genotype chickens by sequencing the BLB2 and BF2 genes of the seven genotype chickens; and by combining the toxin-eliminating result, determining four MD resistance individuals. The established method can detect the MD resistance homozygous genotype chickens, and the MD resistance homozygous genotype chickens are taken as breeders to breed the MD resistance chickens. Therefore, the method has extremely great economic benefits to prevention and control of MD and related immune-suppressing diseases on genetic predisposition.

Description

technical field [0001] The invention belongs to the field of biology, in particular to a detection method for Marek's disease resistance homozygous genotype chickens. Background technique [0002] In the past, breeding of disease-resistant strains was mainly determined by breeding or / and descendants of challenge survivors. The classic example is that Cornell used this method in 1968 for multi-generation selection, and obtained Cornell MD disease-resistant lines and susceptible lines mainly used for scientific research in the fourth generation. These methods are the simplest, but the disadvantages are a large number of basic populations, a special challenge environment, cumbersome progeny determination, and high breeding costs. Contents of the invention [0003] In order to overcome the deficiencies of the prior art, the invention provides a method for detecting Marek's disease resistance homozygous genotype chickens. [0004] The technical solution of the present inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 韦平金元昌韦信贤赵子轶李娅
Owner GUANGXI UNIV
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