Breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content

Abstract

The invention provides a breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content, comprising the following steps: taking exarate pupae breeds (with Nd-s genes) as stuff to be hybridized with the breeds with high amount of silk production, and breeding Japanese special original strains with sericin content being more than 99.0% and homozygous genotypes (Nd-s / Nd-s) by adopting the methods of selfing, backcrossing, marker gene selecting, sericin content tracking and detecting and the like; and hybridizing the Japanese special original strains with the Chinese conventional breeds (Gc / Gc) with green cocoon properties to breed the dedicated silkworm hybrid. The method can provide high quality raw materials to such fields as cosmetics, medicines, food, fiber modified materials and the like and improve the product added value.

Description

technical field

[0001] The present invention relates to the breeding of silkworm varieties with special purposes. Specifically, it uses methods such as hybridization, self-crossing, backcrossing, marker gene selection, trace detection of sericin content, and determination of combining ability to select and breed silkworms that secrete pure natural, high-content, A method for special-purpose silkworm hybrids for green sericin. Background technique

[0002] my country is the largest producer and supplier of cocoon and silk in the world. The output and export volume of cocoon and silk account for more than 70% and 80% of the world's total respectively. Silk is a natural animal protein, mainly composed of two proteins: fibroin in the inner layer and sericin in the outer layer. Silk fibroin is a high molecular weight insoluble fibrin, accounting for 70-80%. The current silk industry mainly uses silk fibroin to make raw silk and silk products; sericin is a soluble globular protei...

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