PCR primer used for amplifying human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence and application thereof

Abstract

The invention relates to a PCR primer for a human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on a NGS technology and application thereof. The PCR primer for the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence comprises at least one capturing primer pair, and sequences of a forward primer and a reverse primer of each capturing primer pair respectively comprise a specific sequence and a linker sequence connected with a terminal 5' of the specific sequence. The PCR primer used for amplifying the human breast cancer susceptibility gene BRCA1 and BRCA2 coding sequence based on the NGS technology and application thereof have the advantages that experiment operations related in the whole method are simple, only a PCR reagent and a primer combination are related, cost is low, and a ditag sequencing linker sequence is introduced for distinguishing different samples, so that high throughput sample sequencing detection can be realized, and gene detection support can be effectively provided for risk assessment on human breast cancer and other BRCA susceptible gene-related hereditary tumours or targeted medication specific to BRCA mutation.

Description

technical field [0001] The invention relates to the field of medical molecular biology, in particular to a PCR primer for amplifying human breast cancer susceptibility genes BRCA1 and BRCA2 coding sequences and its application. Background technique [0002] Breast cancer is the most common cancer in women. According to the "2012 China Cancer Registry Annual Report" data, there are about 220,000 new breast cancer cases in my country every year, accounting for 15% of the world's new breast cancer cases (1.5 million). Among all malignant tumors in women, the incidence rate of breast cancer is as high as 16.8%, about 43 cases per 100,000 people, and the age of onset is younger and the incidence rate is on the rise. The incidence rate rises rapidly at the age of 25 and peaks at the age of 50. Therefore, the high incidence of breast cancer has seriously threatened the health of women in my country. [0003] Familial hereditary breast cancer accounts for 10% to 30% of all breast...

AI Technical Summary

Problems solved by technology

In terms of detection sensitivity, Sanger sequencing can generally only detect mutations with a mutation rate of more than 20%. For the detection of all coding sequences of BRCA1 and BRCA2, the detection throughput is relatively low, the cycle is long, and the cost of single-sample detection is also high.

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