Primer applied to amplicon sequencing library construction and method for constructing amplicon sequencing library

Abstract

The invention discloses a primer applied to amplicon sequencing library construction and a method for constructing an amplicon sequencing library. The primer consists of a forward primer and a reverse primer, and the forward primer and the reverse primer comprise a joint sequence, a sequencing primer sequence and a target fragment amplification primer sequence from a sequence from the 5' end to the 3' end. According to the primer provided by the invention, the joint sequence, the sequencing primer sequence and the target fragment amplification primer sequence are integrated on a pair of primers, so that library construction can be finished in one step by utilizing the primer. The sequencing library quality and library construction efficiency are improved, the constructed amplicon sequencing library can perform high-throughput sequencing due to the joint sequence and the sequencing primer sequence used on a conventional sequencing platform by utilizing a common reagent for on-board sequencing, and the phenomena that an extra sequencing primer is provided and the mixed sequencing primers of the PHiX library are changed are avoided.

Description

technical field [0001] The invention relates to the field of high-throughput sequencing, in particular to a primer suitable for constructing an amplicon sequencing library and a method for constructing the amplicon sequencing library. Background technique [0002] Amplicon sequencing is the sequencing of PCR products or captured fragments of a specific length, mainly including 16S rDNA sequencing, 18S rDNA sequencing, ITS sequencing and functional gene detection. The sequence of a certain hypervariable region of 16S / 18S / ITS determined by the Illumina MiSeq second-generation high-throughput sequencing platform is used to reflect the differences between species in environmental samples in terms of bacterial, fungal, and archaeal classification, which is useful for the study of oceans and soils. The composition of microorganisms in environments such as , intestinal feces, etc. plays an important guiding role; similarly, more biological information can also be mined by sequencin...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a primer suitable for the construction of amplicon sequencing library and a method for constructing amplicon sequencing library, so as to solve the cumbersome steps or easy pollution problems in the existing amplicon sequencing library construction method in the prior art. The defect that the follow-up sequencing on the machine is inconvenient

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