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Kit for classificatory detection of helicobacter pylori

A Helicobacter pylori and kit technology, which is applied in measurement devices, analytical materials, instruments, etc., can solve problems such as HP typing and typing, and achieve the effects of small sample demand, wide application range and good patient compliance.

Inactive Publication Date: 2013-03-13
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the object of the present invention is to provide a test kit for the detection of Helicobacter pylori typing in view of the defect that the detection of HP in the prior art can only detect whether there is HP infection but cannot type HP

Method used

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  • Kit for classificatory detection of helicobacter pylori
  • Kit for classificatory detection of helicobacter pylori
  • Kit for classificatory detection of helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Preparation of Helicobacter pylori (HP) blotting membrane

[0044] 1. HP whole bacterial protein electrophoresis separation

[0045] 1. Prepare the sample buffer: use a sample pipette to draw 10 mL of 0.05M pH7.4 Tris-HCl, 0.5 mL of 2-mercaptoethanol, 2 mL of glycerol, 0.5 mL of 0.1% bromophenol blue, 2 mL of 10% sodium dodecyl sulfate, mix Get evenly.

[0046] 2. Dilute the HP whole cell protein with sample buffer to 0.04±0.005mg / mL, after treatment at 100°C for 3 minutes, set it to cool at 4°C for later use.

[0047] 3. Gradient discontinuous electrophoresis

[0048] 3.1. Preparation of separating gel: measure 40% acrylamide 4mL, 40% methylidene-bisacrylamide 0.1mL, pH9.20.5M Tris-HCL 5mL, DW 5mL, TEMED (tetramethylethylenediamine) 50μL, 1% Ammonium persulfate 5mL, H 2 O 12.5mL.

[0049] 3.2 Glue filling: Measure 30±0.5mL with a graduated cylinder, draw 1mL of n-butanol with a pipette, add it to the glue solution and seal it for solidification.

[005...

Embodiment 2

[0060] Example 2: Kit of the present invention

[0061] A kit for Helicobacter pylori typing and detection, comprising the blotting membrane prepared in Example 1, each sheet having a length of 98±2mm and a width of 150±5mm.

Embodiment 3

[0062] Example 3: Kit of the present invention

[0063] A kit for Helicobacter pylori typing and detection, comprising the blotting membrane prepared in Example 1, an enzyme-labeled antibody and an enzyme substrate, as shown in Table 1.

[0064] Table 1 Kits for Helicobacter pylori typing and detection

[0065]

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Abstract

The invention belongs to the technical field of biology, and discloses a kit for classificatory detection of helicobacter pylori. The kit comprises a blotting membrane transferred with whole helicobacter pylori protein subjected to electrophoretic separation. The kit is used for detecting multiple Hp antibodies such as pathogenic factor cytotoxin (CagA), vacuolating cytotoxin (VacA) and urease subunit A and B antibodies in the serum of a helicobacter pylori infected person by adopting immuno-blotting, determining whether the infected Hp produces strains or not according to different Hp antibodies and further judging the serological types of the Hp strains infecting patients. The kit only requires an examinee to provide the serum, belongs to noninvasive examination, and is high in compliance of the patients, quick in detection (the whole time is only 2 hours), low in demand of samples (only 10 milliliters of examined serum is required), high in specificity, high in sensitivity, wide in application range and convenient to popularize and use in hospitals and hygiene departments.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for Helicobacter pylori typing and detection. Background technique [0002] Helicobacter Pylori, referred to as HP, was first isolated from gastric mucosa in 1983 by Australian scholars Barry Marshall and Robin Warren. After years of research by scholars from various countries, it has been confirmed that Hp plays an important role in the pathogenesis of chronic gastritis, peptic ulcer, gastric cancer and other digestive tract diseases. The discovery of HP was a revolutionary development in the history of gastroenterology, for which Robin Warren and Barry Marshall were awarded the Nobel Prize in Medicine in 2005. [0003] The relationship between HP and gastroduodenal diseases has always been the main subject of research by scholars from all over the world. A large number of epidemiological investigations have confirmed that the infection rate of HP in the natural ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/544
Inventor 马伟民张永顶马新民
Owner SHENZHEN BLOT BIOTECH
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