System for detecting multiple quantitative and virulent genes of H.pylori as well as kits and applications of system

A technology for Helicobacter pylori and gene detection, applied in the biological field, can solve the problems of low sensitivity, small flux, long time, etc., and achieve the effects of avoiding mutual interference, making up for low flux, and reducing false positives.

Active Publication Date: 2016-04-20
HUADONG HOSPITAL +1
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that it does not reflect current infection
5) Quantitative analysis: real-time PCR is commonly used, but this method has a single detection, low throughput, and high cost when analyzing m...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • System for detecting multiple quantitative and virulent genes of H.pylori as well as kits and applications of system
  • System for detecting multiple quantitative and virulent genes of H.pylori as well as kits and applications of system
  • System for detecting multiple quantitative and virulent genes of H.pylori as well as kits and applications of system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. The composition of the kit

[0051] The Helicobacter pylori detection kit of this example includes: primer mixture, PCR buffer (10×PCRBuffer), MgCl 2 solution, dNTPs, fluorescent Universal Labeling Mix, hot-start DNA polymerase (TaqDNA Polymerase), positive and negative controls. 2mmol / L dNTPs and fluorescent universal labeling mixture are mixed together into a tube of reagents.

[0052] PCR buffer, dNTPs and hot-start DNA polymerase were all from Takara (Catalog No.: R007A).

[0053] The positive control solution is a plasmid mix that includes all gene targets of interest.

[0054] The negative control solution was nuclease-free ultrapure water.

[0055] The primer mixture includes a forward primer for the 16SrRNA gene, a reverse primer for the 16SrRNA gene, a forward primer for the cagA gene, a reverse primer for the cagA gene, a forward primer for the vacA-s1 or vacA-s2 gene, Reverse primer for vacA-s1 or vacA-s2 gene, forward primer for vacA-m1 gene, reverse ...

Embodiment 2

[0114] The Helicobacter pylori detection kit of this example is the same as the rest of Example 1, except that the primer mixture only includes the forward primer for the 16SrRNA gene, the reverse primer for the 16SrRNA gene, and the forward primer for the cagA gene. Primers, Reverse primer for cagA gene, Forward primer for vacA-s1 or vacA-s2 gene, Reverse primer for vacA-s1 or vacA-s2 gene, Forward primer for vacA-m1 gene, Forward primer for vacA - Reverse primer for m1 gene, forward primer for vacA-m2 gene, reverse primer for vacA-m2 gene, forward primer for iceA1 gene, reverse primer for iceA1 gene, forward primer for iceA2 gene Primer, reverse primer for iceA2 gene, forward primer for dupA gene, reverse primer for dupA gene, forward primer for oipA gene, reverse primer for oipA gene, forward primer for luxS gene, Reverse primer for luxS gene, forward primer for ureC gene, reverse primer for ureC gene, forward primer for ?-globin gene, reverse primer for ?-globin gene.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a system for detecting multiple quantitative and virulent genes of H.pylori as well as kits and applications of the system. The system for detecting multiple quantitative and virulent genes of H.pylori comprises multiple pairs of primers, each for strain identification genes (16S rRNA), quantitative analysis genes ureCand Beta-globin, as well as virulent genes (cagA, vacA-s1, vacA-s2, vacA-m1, vacA-m2, iceA1, iceA2, dupA, oipA and luxS). The system for detecting multiple quantitative and virulent genes of H.pylori and the kits of the system can directly perform synchronous detection and analysis on the strain identification, quantification and virulence of a tissue sample in a same reaction system without adopting a conventional culture step or other steps, remedy defects that a conventional detection method is low in throughput, time-consuming and low in detection rate, obviously improve the accuracy of detection results, immediately provide a comprehensive, accurate and low-cost etiological diagnosis for clinic, and provide an important reference for the accurate diagnosis, differential diagnosis and disease prognosis of H.pylori infection.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic, campylobacter that mainly resides in the human stomach. Helicobacter pylori infection is closely related to the occurrence and development of chronic atrophic gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer, so it has attracted widespread clinical attention. In 1994, the International Agency for Research on Cancer (IARC) listed it as a human I carcinogen, and it is the only bacterial pathogenic microorganism listed as a clear carcinogen to humans so far. Many reports believe that Helicobacter pylori infection is related to diseases such as coronary heart disease, rheumatoid, hepatobiliary disease, tuberculosis, pregnancy vomiting, colorectal cancer and various skin d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6851C12Q1/689C12Q2600/16
Inventor 张艳梅赵虎赵付菊胡彬婕王诗雯陈飞吴勇缪应新张景皓姜文荣徐玲丽南丽
Owner HUADONG HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap