Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Helicobacter pylori IgG antibody ELISA semi-quantitative detection kit and application thereof

A semi-quantitative detection technology for Helicobacter pylori, applied to measuring devices, instruments, scientific instruments, etc., to achieve high consistency of results

Inactive Publication Date: 2015-08-12
BIOHIT BIOTECH HEFEI
View PDF7 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But unfortunately, all kinds of Helicobacter pylori IgG antibody ELISA detection kits in the domestic and foreign markets are qualitative tests, and the IgG antibody content has an important prompting effect on the colonization density of Helicobacter pylori and the judgment of the efficacy of Helicobacter pylori eradication

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Helicobacter pylori IgG antibody ELISA semi-quantitative detection kit and application thereof
  • Helicobacter pylori IgG antibody ELISA semi-quantitative detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] 1. Reagents

[0032] (1) Coating solution: 0.05mol / L, sodium carbonate-sodium bicarbonate buffer solution with pH 9.6,

[0033] Na 2 CO 3 1.5g, NaHCO 3 2.9g, Na 2 N 3 0.2g, add double distilled water to make up to 1000ml, adjust to pH9.45-9.75;

[0034] (2) Coating stabilization solution: an aqueous solution of 1% casein+5% sucrose,

[0035] Sucrose 5g, casein 1g, add double distilled water to 1000ml to dissolve, adjust the pH value to 7.15-7.25;

[0036] (3) Blocking solution: 10% calf serum + 0.02mol / L PBS (pH7.4)

[0037] Add 100ml calf serum to the prepared 0.02mol / L phosphate buffer solution to dissolve and quantify to 1000ml;

[0038] (4) Specimen diluent: 10% bovine serum albumin+0.01mol / L phosphate buffered saline PBS (pH7.4), 100g calf serum, 8.0gNaCl, 0.2gKH 2 PO 4 , 2.9gNa 2 HPO 4 12H 2 O, 0.2g KCl, 0.1g thimerosal, 1g preservative ProClin300, add double distilled water to make the volume to 1000ml, adjust to pH7.4, to get final product;

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention provides a helicobacter pylori IgG antibody ELISA semi-quantitative detection kit. The kit is formed by a helicobacter pylori antigen coated enzyme labeled reaction plate, a sample diluting solution, a washing liquid, an enzyme labeled second antibody, a substrate solution, a stopping solution, a positive control solution, a negative control solution and a standard solution. A method for rapid and sensitive ELISA semi-quantitative detection of a helicobacter pylori specific IgG antibody in serum is established by using the kit. The helicobacter pylori IgG antibody standard solution is prepared, the helicobacter pylori antigen coated enzyme labeled reaction plate is prepared based on the standard solution, an indirect ELISA method is adopted to detect the specific helicobacter pylori IgG antibody in the serum, and the specific helicobacter pylori IgG antibody is quantified through calculation of an enzyme immunity unit (EIU). The kit has the advantages of high specificity and high sensitivity, and is mainly used for laboratory researches and clinic auxiliary diagnosis.

Description

technical field [0001] The invention relates to a biological detection kit, in particular to an ELISA semi-quantitative detection kit for Helicobacter pylori IgG antibody, which can be used for rapid detection of Helicobacter pylori IgG antibody in serum and semi-quantitative calculation of IgG antibody content. box. Background technique [0002] Helicobacter pylori (H.p), first discovered by Barry J.Marshall and J.Robin Warren, who won the 2005 Nobel Prize in Physiology or Medicine prize. Helicobacter pylori is a unipolar, multi-flagellate, blunt-ended, spirally curved bacterium, 2.5-4.0 μm long and 0.5-1.0 μm wide. On the surface of gastric mucosal epithelial cells, it often presents a typical spiral or arc shape. [0003] Helicobacter pylori infection is the main pathogenic factor of chronic active gastritis, peptic ulcer and gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and is closely related to the occurrence of gastric cancer. In 1994, the World Health...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56911G01N33/5436G01N2333/20
Inventor 刘峰
Owner BIOHIT BIOTECH HEFEI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com