Reagent strip for joint detection of syphilis specific IgG antibody and specific total antibody and preparation method thereof

Abstract

The invention provides a reagent strip for joint detection of a syphilis specific IgG antibody and a specific total antibody and a preparation method thereof, relating to a reagent for joint detection of a syphilis specific IgG antibody and a specific total antibody. The reagent strip is provided with two reagent strips, wherein one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a syphilis specific IgG antibody detection line, a control line and an absorbent pad; and the other one is provided with a vector plate, a sample adding pad, a colloidal gold pad, a nitrocellulose membrane, a specific total antibody detection line, a control line and an absorbent pad. The preparation method comprises the following steps: preparing recombinant syphilis antigens TPN17 and TPN47, applying samples of nitrocellulose membranes, preparing colloidal gold, labeling TPN17 and TPN47 with colloidal gold and preparing immunochromatography detection strips. The reagent strip can be used for detecting the syphilis specific IgG antibody and the specific total antibody in the specimens of whole blood, blood serum, blood plasma, cerebrospinal fluid and the like. During detection, the specimen amount is small, special instruments are not needed and the results are directly interpreted by the naked eyes. The reagent strip is simple, convenient, fast, accurate and reliable and has strong specificity and high sensitivity.

Description

Syphilis-specific IgG antibody and specific total antibody combined detection reagent strip and preparation method thereof technical field The invention relates to a combined detection reagent for syphilis-specific IgG antibodies and specific total antibodies, in particular to a reagent strip for combined detection of syphilis-specific IgG antibodies and specific total antibodies using colloidal gold immunochromatography (immunochromatography) and its preparation method. Background technique Syphilis is a sexually transmitted disease caused by Treponema pallidum (TP), and its pathogen is Treponema pallidum, which belongs to the family Treponemaceae. Treponema pallidum is mainly transmitted through sexual contact, blood transfusion, wound or placenta. Treponema pallidum enters the blood from the lymph nodes near the infected area, spreads throughout the body, and affects almost all tissues and organs of the body. The World Health Organization (WHO) once predicted optimist...

AI Technical Summary

Problems solved by technology

Although a negative TP-IgM does not completely rule out infectivity, a positive TP-IgM must suggest that the patient is infectious

Images