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Method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine

A technology of dried blood spots and amino acids, which is applied to forty kinds of amino acids in blood and urine, simultaneously detects the field of dried blood spots, and achieves the effects of avoiding distortion, high throughput and easy operation.

Inactive Publication Date: 2018-07-27
JINAN YING SHENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no effective method for the simultaneous detection of more than 40 underivatized amino acids

Method used

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  • Method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine
  • Method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine
  • Method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine

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Experimental program
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Effect test

Embodiment

[0079] (1) Prepare the sample to be tested

[0080] a Dried blood spot sample treatment: punch three round holes with a diameter of 3.2 mm on the dried blood spot and dissolve it in water for 20 minutes, and then centrifuge to get the supernatant, and add an equal volume of methanol:acetonitrile (volume ratio of 1 to the supernatant). : 1) solution, centrifuged after vortex shaking, took the supernatant into a sample bottle, blown dry with nitrogen gas, redissolved the sample in 0.1% tridecafluoroheptanoic acid aqueous solution, filtered through a 0.2 μm filter membrane, and obtained the sample to be tested;

[0081] b Serum sample treatment: Add an equal volume of methanol:acetonitrile (volume ratio 1:1) solution to the serum sample, vortex and shake, then centrifuge, take the supernatant into a sample bottle, blow dry with nitrogen, and redissolve the sample in 0.1 % tridetrifluoroheptanoic acid aqueous solution, 0.2μm filter membrane filtration, to obtain the sample to be t...

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Abstract

The invention discloses a method for simultaneously detecting forty types of amino acids in dried blood spots, blood and urine. The method comprises the following steps of firstly, pretreating a biological sample by a simple liquid and liquid extracting method; performing chromatographic separation and mass spectrometric detection; using the relative retention time and qualitative ions as the qualitative basis for each type of amino acid, and using a standard product to manufacture a standard curve for quantifying. The method has the advantages that the accuracy and validity of the method shall be studied by three levels of quality control products, so as to avoid the distortion of detection results; the forty types of non-derived amino acids in one sample can be simultaneously detected bya HPLC-MS / MS (high performance liquid chromatography-mass spectrograph) technique for the first time; the operation is simple, convenient and rapid, the flux is high, and the cost is low; the level of amino acids in a human body can be effectively monitored in real time; the enough basis is provided for the clinical disease diagnosis; the method is suitable for being clinically popularized and applied.

Description

technical field [0001] The invention belongs to the detection field of amino acids, in particular to a method for simultaneously detecting forty amino acids in dried blood spots, blood and urine. Background technique [0002] Amino acid is the cornerstone of life, the basic nutrient for the human body, and is closely related to the life activities of organisms. Amino acid is not only the basic unit of protein, but also an important nitrogen source for the synthesis of nucleic acid and provides energy. There are more than 180 kinds of amino acids that have been found in nature, and there are more than 40 kinds of important amino acids in the human body, including 10 kinds of essential amino acids, more than 20 kinds of amino acids that make up proteins and some amino acids with important physiological significance, collectively called full-spectrum amino acids. [0003] The full-spectrum amino acid is a balanced state in the body. The imbalance of the full-spectrum amino aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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