Quantitative detection device and detection method of biological fluid samples

Abstract

The invention discloses a quantitative detection device of biological fluid samples, which comprises a test strip arranged in a box body, a color intensity data memory and a color intensity data reader, wherein a substrate is arranged at the bottom layer of the test strip, and a porous test main film is arranged on the substrate; a second film is arranged at the upstream of the test main film, a sample application site and a tagged ligand site are arranged on the second film, and a biological fluid sample to be tested and compounds thereof and the tagged ligand can be promoted under the capillary action to flow; a third film is arranged at the downstream of the test main film, and the third film is used for adsorbing the biological fluid sample to be tested passing through the test main film at the downstream end of the test main film; and two sample injecting holes and a viewing window are arranged on the box cover of the box body. The detection device of the invention can be used for drawing a standard sample curve through the light reflection intensity of the standard sample with known concentration, thereby quickly, effectively and accurately carrying out quantitative analysis on the biological body fluid sample.

Description

technical field [0001] The invention relates to a system for detecting biological fluid samples, in particular to a method for detecting and quantitatively measuring the concentration of analyte in the sample. Background technique [0002] Quantitative analysis of antigens, hormone-like antibodies, endocrine proteins, and other types of proteins often provides crucial clinical data. In the biological field, immunoassays are well known. The principle is that after the reagents are impregnated, they are capillary-action on the membrane containing the immobilized reagents, and the visible markers, such as latex particles and metal complexes, are bound to the detection zone of the test strip. reaction of the analyte. It is worth mentioning that the label is obtained from the metal sol, and the label processed from the metal sol can be combined with the ligand, and then further combined with the analyte, ligand or antibody. Test strips have been used extensively for the quantit...

AI Technical Summary

Problems solved by technology

[0005] In the prior art, most of the devices for determining the presence or absence of the analyte based on the test strip cannot provide the accurate amount of the analyte in the liquid sample.

Even if the detection device is equipped with a quantitative test standard, it will be inaccurate due to temperature, air humidity, time lapse, test strip differences, and signal intensity changes

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