Detection kit and detection method of circulating tumor cells

Abstract

The invention discloses a detection kit and a detection method of circulating tumor cells. The kit comprises a PBS buffer solution, a density gradient separation medium, a red blood cell lysate, a CD45 immunomagnetic bead, an incubation solution, a fixing solution, a permeabilizing solution, a confining liquid, an antifade mounting medium, an antibody dilution solution and a fluorescent antibody concentrate, and the fluorescent antibody concentrate contains a fluorescent dye pan-CK-AF488, a fluorescent dye CD45-PE and a cell nucleus dye Hoechst33342. The detection method comprises the following steps: S10, centrifuging a blood sample, and separating and enriching the CTCs; and S20, carrying out dyeing identification on the enriched CTCs through the cell nucleus dye pan-CK-AF488, the fluorescent dye CD45-PE and Hoechst33342 fluorescence antibodies, wherein the detection is carried out for the purpose of non-treatment. When the detection kit and the detection method are adopted to perform detection, the detection result is accurate and reliable.

Description

technical field [0001] The invention relates to the research field of in vitro diagnostic technology, in particular to a detection kit and a detection method for circulating tumor cells. Background technique [0002] The World Health Organization predicts that malignant tumors will become the "first killer" of human beings in the 21st century, so cancer control has become the focus of global health strategies. Although my country is a developing country, the spectrum of diseases has changed. According to reports, in 2012, the number of cancer patients in my country was 3.065 million, accounting for about one-fifth of the global incidence; the number of cancer deaths was 2.205 million, accounting for about 1 / 5 of the global cancer deaths. my country has become the world's largest cancer country. According to epidemiological surveys, in recent years, there are about 1.6 million new cases and 1.3 million deaths in my country every year. On average, one person dies of cancer for...

AI Technical Summary

Problems solved by technology

However, the above staining methods still have problems such as easy antigen degradation, poor fluorescent staining effect, easy missed detection, difficulty in multiple staining analysis, cumbersome operation, low throughput, and difficulty in counting CTCs before and after treatment to evaluate the therapeutic effect.

At present, the above separation and identification methods are difficult to achieve satisfactory analysis purposes, and it is urgent to establish an efficient enrichment and identification method

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