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IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method

A magnetic sorting and immune magnetic bead technology, applied in stress-stimulated microbial growth methods, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as difficulty in miniaturization, reduced operational reliability, and unsatisfactory sealing conditions, etc. Achieve high repeatability, prevent sample leakage, and compact structure

Inactive Publication Date: 2011-04-06
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the several automatic magnetic sorters mentioned above, RoboSep and BeadRetriever can only run automatically for a limited number of times (RoboSep is 4 times, BeadRetriever is 15 times) after adding a sample, and then the consumables need to be replaced manually. It cannot run continuously for a long time when unattended; the mechanical movement structure is used inside the instrument, the operation reliability is reduced, and it is not easy to realize miniaturization
The processing of samples and reagents in RoboSep and BeadRetriever is carried out in an open or semi-open form, which does not meet the sealing conditions; the sample liquid after sorting stays in the test tube, which is inconvenient to seamlessly connect with other subsequent processing equipment
In summary, none of the existing magnetic sorting equipment can fully meet the needs of automatic sorting on-site or unattended.

Method used

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  • IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method
  • IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method
  • IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method

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Embodiment Construction

[0038] In order to further understand the invention content, characteristics and effects of the present invention, the following examples are given, and detailed descriptions are as follows in conjunction with the accompanying drawings:

[0039] The overall structure of the embodiment of the sequential flow automatic magnetic separation device based on immunomagnetic beads is as follows: figure 1 As shown, the device includes a control system and a hydraulic system, wherein the control system is mainly composed of a main control module 1, a precision injection pump 5, an electromagnetic three-way valve 7, an electric multi-position valve 3 and an electromagnet 14; the hydraulic system is mainly composed of The sample injection column 17, the magnetic bead column 16, the mixing column 15, the magnetic separation column 13, the sample output column 12, the cleaning liquid bottle 9, the waste liquid bottle 10, the transfer pipe 4 and the pipeline 11 connecting the components. In ...

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Abstract

The invention relates to the technical field of magnetic separation on biological samples. The invention discloses an IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device, which comprises a control system and a fluid-channel system, wherein the control system comprises a master control module, a precise injection pump, an electromagnetic three-way valve, an electrically operated multi-position valve and an electromagnet; the fluid-channel system comprises a sample feeding column, a magnetic bead column, a mixed column, a magnetic separation column, a sample discharge column, a cleanout fluid bottle, a spent liquor bottle, an interim tube and a pipeline communicated with the above components; the master control module is used for controlling the precise injection pump, the electromagnetic three-way valve, the electrically operated multi-position valve and the electromagnet to execute corresponding actions according to a preset process and a preset time sequence, so that the samples to be separated and related reagents are transferred and processed in the fluid-channel system according to a certain sequence. The invention also discloses a method for carrying out automatic magnetic separation by using the above device. The device and the method of the invention can satisfy the requirements for field or unattended automatic magnetic separation.

Description

technical field [0001] The invention belongs to the technical field of biological sample sorting, in particular to the technical field of magnetic sorting by immunomagnetic beads, and specifically relates to a sequential flow automatic magnetic sorting device and method using immunomagnetic beads. Background technique [0002] The magnetic separation technology based on immunomagnetic beads is one of many current biological sample separation technologies. Its basic principle is to couple specific antibodies to microbeads with superparamagnetic properties and good dispersion to form immunomagnetic beads. Then use the antibody coupled to the immunomagnetic beads to identify and label the antigens on the surface of the target cells (or other biological samples) with high specificity, and then use the magnetic separation technology to separate the immunomagnetic beads together with the labeled cells (or other biological samples) Biological samples) were separated from the origin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
CPCC12M27/00C12M47/04C12M35/02
Inventor 杜耀华吴太虎陈锋程智顾彪
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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