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Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique

A technology of luminescence immunoassay and analysis system, which is applied in the field of small-scale automatic luminescence immunoassay system, which can solve the problems of undiscovered fully automatic luminescence immunoassay system, etc., so as to save the steps of suction and discharge, reduce the volume and cost of the system, and increase the sensitivity Effect

Active Publication Date: 2014-02-26
THE SECOND AFFILIATED HOSPITAL OF NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports of using commercially available nucleic acid extraction equipment to complete the magnetic enzyme immunoreaction, and finally manually realizing the luminescence detection in the microplate reader, but no system has been found to realize the automatic immunoluminescence analysis by using the magnetic bead transfer method

Method used

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  • Full-automatic luminescent immunoassay system based on micronano-magnetic bead electromagnetic transfer technique

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Embodiment 1

[0029] 1. Self-inspection and preparation stage

[0030] Such as figure 1 As shown, start the preheating self-test, set the temperature of the temperature control device 8 to be stable at 37°C, set the blood dilution sample to be tested into the sample pool 7, put the corresponding reagent chamber consumables 13 into the consumables adapter 11, Placed in a fixed location on the system. The box is sealed, and the membrane rupture device 3 is driven by the first motor 1 to first crush the encapsulation membrane on the consumables 13 of the kit along the guide rail 14 .

[0031] 2. Immunomagnetic beads capture sample stage

[0032] Driven by the second motor 2, the electromagnetic stirring bar 4 moves along the guide rail 14 to the top of the container in which the immunomagnetic beads 6 are stored in the consumables 13 of the reagent kit, and then is driven by the second motor 2 to penetrate into the reagent chamber, and stir and mix the immunomagnetic beads 6 evenly. Bead 6 ...

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Abstract

The invention provides a full-automatic luminescent immunoassay system based on a micronano-magnetic bead electromagnetic transfer technique. The system includes a computer and a detection box. The detection box is a sealable lightproof box, and comprises a first motor, a second motor, a membrane rupture device, an electromagnetic stirring rod, a Teflon coating or a disposable plastic jacket, immunomagnetic beads, a sample pool, a temperature control device, a photoelectric conversion device, a bottom reading light path, a waste consumable material recovery area or ultrasonic cleaning equipment, a reagent bin consumable material and a consumable material adapter, a guide rail and an electromagnet. The system provided by the invention is based on a magnetic bead transfer method, simplifies the detection steps, integrates the detection module, avoids a complex liquid path system, saves the detection time while realizing full automation and miniaturization of the immunodetection system, and also lowers the system cost at the same time.

Description

technical field [0001] The invention belongs to the field of medical instruments, in particular to a small-sized fully automatic luminescent immunoassay system required by laboratory medicine in mobile hospitals. Background technique [0002] The fully automatic immune analysis system can generally and significantly improve the specificity of immune experiments and the sensitivity of reagents, and reduce the errors caused by human factors. Fully automatic enzyme immunoassay equipment has been widely used in clinical practice. Based on the principle of ELISA detection, the main classifications are as follows according to the different solid-phase carriers bound by antibodies: [0003] (1) Container-type solid-phase carriers, which can be subdivided into microplates, plastic tubes, plastic gun tips, etc., when antibodies are bound to such solid-phase carriers, the instrument automatically performs pipetting, incubation, cleaning, etc. process, due to the steric hindrance of a...

Claims

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Application Information

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IPC IPC(8): G01N35/00G01N35/10G01N33/53
Inventor 司进李智洋
Owner THE SECOND AFFILIATED HOSPITAL OF NANJING MEDICAL UNIV
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