Immunomagnetic beads preservation solution

Abstract

The invention discloses an immunomagnetic beads preservation solution, which comprises a buffer, an inert protein, glycerin, a water-soluble sugar, a synthetic high molecular polymer, a protein stabilizer, a reducing substance, an amino acid, a chelating agent, a serum, a surfactant, and a preservative. According to the immunomagnetic beads preservation solution of the invention, the activity of immunomagnetic beads can be maintained at a high level within one year at 4 DEG C storage conditions, thereby providing a guarantee for the practical detection of the immunomagnetic beads.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, in particular to an immunomagnetic bead preservation solution. Background technique [0002] Immunomagnetic beads (IMBS) chemically modify the surface of superparamagnetic particles to make them firmly bind to specific antibodies and become magnetic beads that can capture specific antigens. [0003] Mix the immunomagnetic beads with the solution to be tested. If there is a corresponding antigen, the immunomagnetic beads will capture it, form an antigen-immune magnetic bead complex, and separate it under appropriate magnetic field conditions to achieve the goal of enriching the target antigen. Purpose. [0004] As a specific separation and enrichment technology, immunomagnetic beads have important application value in many fields, such as DNA extraction, protein purification, cell screening, enrichment of food-borne pathogenic microorganisms, etc. Immunomagnetic beads are used mo...

AI Technical Summary

Problems solved by technology

[0008] Commercial immunomagnetic bead preservation solutions are rarely sold on the market, and conventional preservation solutions are generally PBS or Tris-HCl buffer solution with bacteriostats and 1% BSA added, and the preservation period is only about ten days, and the preservation effect cannot be improved. people are satisfied

In addition, during use, it was found that due to the different conjugated antibodies, there are certain differences in the preservation effect of the preservation solution, and the versatility is poor.

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