Immunomagnetic beads preservation solution

A technology of immunomagnetic beads and preservation solution, applied in the field of microbial detection, can solve the problems of poor versatility, unsatisfactory preservation effect, difference in preservation effect of preservation solution, etc., and achieve the effect of high activity

Active Publication Date: 2019-05-07
GUANGDONG HUANKAI MICROBIAL SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Commercial immunomagnetic bead preservation solutions are rarely sold on the market, and conventional preservation solutions are generally PBS or Tris-HCl buffer solution with bacteriostats and 1% BSA added, and the preservation period is only about ten days, an

Method used

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Embodiment Construction

[0030] An immunomagnetic bead preservation solution contains buffer, inert protein, glycerin, water-soluble sugar, artificially synthesized high molecular polymer, protein stabilizer, reducing substance, amino acid, chelating agent, serum, surfactant and preservative.

[0031] As a further improvement of the above immunomagnetic bead preservation solution, the concentration of each component of the preservation solution is: 10-100g / L inert protein, 5-30g / L glycerol, 40-150g / L water-soluble sugar, 1-25g / L artificial Synthetic polymer, 50.5~205g / L protein stabilizer, 9~60g g / L reducing substance, 2~25g / L amino acid, 0.5~12g / L chelating agent, 0.5~7v / v% serum, 2~ 15g / L surfactant, appropriate amount of preservatives.

[0032] The main function of the inert protein is to maintain antibody activity. As a further improvement of the above-mentioned immunomagnetic bead preservation solution, the inert protein is at least one of BSA and sodium caseinate.

[0033] As a further improvem...

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Abstract

The invention discloses an immunomagnetic beads preservation solution, which comprises a buffer, an inert protein, glycerin, a water-soluble sugar, a synthetic high molecular polymer, a protein stabilizer, a reducing substance, an amino acid, a chelating agent, a serum, a surfactant, and a preservative. According to the immunomagnetic beads preservation solution of the invention, the activity of immunomagnetic beads can be maintained at a high level within one year at 4 DEG C storage conditions, thereby providing a guarantee for the practical detection of the immunomagnetic beads.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, in particular to an immunomagnetic bead preservation solution. Background technique [0002] Immunomagnetic beads (IMBS) chemically modify the surface of superparamagnetic particles to make them firmly bind to specific antibodies and become magnetic beads that can capture specific antigens. [0003] Mix the immunomagnetic beads with the solution to be tested. If there is a corresponding antigen, the immunomagnetic beads will capture it, form an antigen-immune magnetic bead complex, and separate it under appropriate magnetic field conditions to achieve the goal of enriching the target antigen. Purpose. [0004] As a specific separation and enrichment technology, immunomagnetic beads have important application value in many fields, such as DNA extraction, protein purification, cell screening, enrichment of food-borne pathogenic microorganisms, etc. Immunomagnetic beads are used mo...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/569
Inventor 曲晓莹吴清平蔡芷荷卢勉飞滕昆仑李泽康林干
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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