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Preparation method of human DC (dendritic cell) tumor vaccine

A technology for dendritic cells and tumor vaccines is applied in the field of preparation of human dendritic cell tumor vaccines, which can solve problems such as not achieving very ideal effects, and achieve the effects of being beneficial to treatment, simple in preparation process, and optimized in preparation process.

Inactive Publication Date: 2019-03-15
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are also some researches on DC tumor vaccines in China. However, the preparation methods of DC tumor vaccines that have been disclosed so far have not achieved very satisfactory results in clinical application and need to be further improved.

Method used

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  • Preparation method of human DC (dendritic cell) tumor vaccine

Examples

Experimental program
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Embodiment 1

[0029] Preparation of Example 1 Human Dendritic Cell Vaccine and Detection of Maturity

[0030] The first step: further isolate and obtain monocytes from the isolated PBMCs, including the following steps:

[0031](1) 50 mL of peripheral venous blood was collected from the patient, and mononuclear cells were obtained by density gradient centrifugation of polyscan-diatrizoate glucosamine. The specific steps are: 1500 rpm, centrifuge for 10 minutes, absorb the upper plasma layer, inactivate at 56°C for 30 minutes and then centrifuge for later use, dilute the precipitated blood cells with normal saline, human lymphocyte separation medium and diluted blood at 1:2 The proportion of the mixture was added to the centrifuge tube, 2000 rpm, centrifuged for 20 minutes, carefully absorbed from the membrane layer, washed twice with normal saline, the rotation speed was 1600 rpm, 1300 rpm, and both were centrifuged for 7 minutes to obtain the peripheral Blood mononuclear cells.

[0032] (...

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Abstract

The invention belongs to the field of cellular immunology and particularly relates to a preparation method of a human DC (dendritic cell) tumor vaccine. Specifically, differentiated but not mature monocytes of DCs are induced by a DC maturity promoting agent to become mature DCs, wherein the DC maturity promoting agent is a DC maturity promoting agent combination of IL-1 beta, TNF-alpha, IFN-gammaand human recombinant protein gp100. DC purity and maturity of the human DC tumor vaccine prepared with the method are improved greatly, fusion of the DCs with a tumor antigen is promoted, and tumortreatment is facilitated; the preparation process is simple, and the cost is low.

Description

technical field [0001] The invention belongs to the field of cellular immunology, and in particular relates to a preparation method of a human dendritic cell tumor vaccine. Background technique [0002] The current conventional tumor treatment methods include surgical treatment, radiotherapy and chemotherapy, etc. The main problem faced by these treatment methods is the high rate of tumor recurrence and metastasis. The reason is that the tumor stem cells in the tumor are not all killed, which makes it easier for the cancer tissue to regenerate and re-form the tumor. Dendritic cells (DC, Dendritic Cell) are known to be the most powerful professional antigen-presenting cells in the human body, and have the function of activating T cells and killing tumor cells. Figuratively speaking, DC is equivalent to the messenger between tumor antigens and T cells, and can transmit information between the two parties. In particular, DCs sensitized with different forms of tumor antigens i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784A61K39/00A61P35/00
CPCA61K39/0011A61K2039/5154A61P35/00C12N5/0639C12N2500/84C12N2501/22C12N2501/2301C12N2501/25C12N2506/115
Inventor 宁永玲戚春建丁骏
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
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