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Application of cistanche deserticola polysaccharides as vaccine adjuvant

A technology of Cistanche deserticola polysaccharide and vaccine adjuvant, applied in the field of immune adjuvant, can solve the problems of side effects, induced antibody response, etc., and achieve the effects of small side effects, simple preparation process and wide sources.

Inactive Publication Date: 2015-11-25
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many raw materials can be used in vaccine adjuvants, most of them can only induce antibody responses or produce some side effects, thus limiting its practical application
At present, there are few types of immune adjuvants that can be used clinically. The only FDA-approved adjuvant that can be used in humans - aluminum gel adjuvant can only stimulate humoral immunity, but cannot induce cell-mediated immune responses, and It is ineffective against antigens such as human immunodeficiency virus (HIV), hepatitis C virus (HCV), and influenza virus, so research on screening new vaccine adjuvants that can be used in humans and animals with safety, high efficiency, and no toxic side effects has attracted increasing attention

Method used

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  • Application of cistanche deserticola polysaccharides as vaccine adjuvant
  • Application of cistanche deserticola polysaccharides as vaccine adjuvant
  • Application of cistanche deserticola polysaccharides as vaccine adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Animal experiment of different doses of Cistanche deserticola polysaccharides on mouse immune model antigen ovalbumin (OVA)

[0026] The preparation method of present embodiment Cistanche deserticola polysaccharide is:

[0027] Step 1: Degrease fresh or dried Xinjiang Cistanche powder with petroleum ether, add distilled water for ultrasonication, add absolute ethanol to stir and precipitate the filtrate after concentration, centrifuge the precipitate, and vacuum dry to obtain the crude product of Cistanche deserticola polysaccharide;

[0028] Step 2: Use Sevage reagent (chloroform:n-butanol ratio: 4:1) to remove protein from cistanche cistanche, centrifuge, combine the supernatant, repeat the above operation several times, collect the supernatant, add ethanol, let stand overnight, and centrifuge the precipitate , and vacuum-dried to obtain the polysaccharide of Cistanche deserticola.

Embodiment approach

[0030] Test material: Cistanche polysaccharide mother liquor Configuration: 0.1g of Cistanche deserticola polysaccharide is dissolved in 10ml of normal saline to prepare a 10mg / ml mother liquor. Prepare OVA mother solution: dissolve 0.01g OVA in 10ml of normal saline, and prepare a 1mg / ml mother solution.

[0031] Animal immunization: ICR mice aged 6-8 weeks were randomly assigned to 7 groups, 6 mice in each group, and the experimental groups were shown in Table 1.

[0032] Table 1 Immune grouping of mice

[0033] Group No species (female) only count Injectable ingredients 1 ICR 6 0.9%NaCl 2 ICR 6 AECD 400 μg 3

6 OVA 10 μg 4 ICR 6 Alum200 μg+OVA 10 μg 5 ICR 6 AECD20μg+OVA 10μg (low dose) 6 ICR 6 AECD 100 μg+OVA 10 μg (medium dose) 7 ICR 6 AECD 400 μg+OVA 10 μg (high dose)

[0034] Each group of vaccines was dissolved in normal saline, and each mouse was injected with 100 microliters, subcutan...

Embodiment 2

[0053] Example 2: Cistanche deserticola polysaccharide adjuvant to immune model animal experiment of influenza virus inactivated vaccine (Flu)

[0054] The preparation of Cistanche deserticola polysaccharides in this example was prepared according to the method described in Example 1 of the present invention.

[0055] Test material: Cistanche cistanche multipond was prepared as in Example 1, and the influenza vaccine was commercially available.

[0056] Animal immunization: female ICR mice aged 6-8 weeks were divided into 6 groups, 6 mice in each group, and the experimental groups were shown in Table 6.

[0057] Table 6 Mouse immune groups

[0058] Group No species (female) only count Injectable ingredients 1 ICR 6 0.9%NaCl 2 ICR 6 AECD800μg 3 ICR 6 Influenza vaccine 0.5μg 4 ICR 6 Influenza vaccine 0.5μg+AECD100μg 5 ICR 6 Influenza vaccine 0.5μg+AECD400μg 6 ICR 6 Influenza vaccine 0.5μg+AECD800μg

[00...

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Abstract

The invention provides application of cistanche deserticola polysaccharides as a vaccine adjuvant. Through tests such as detecting OVA (ovalbumin) specific antibody titer and cellular immunological activity in mice sera by animal grouping and immunizing methods and ELISA (enzyme linked immunosorbent assay), results show that the cistanche deserticola polysaccharides can serve as the adjuvant of a protein vaccine, a nucleic acid vaccine, a polypeptide vaccine, an inactivated vaccine or a live attenuated vaccine, and are obviously superior to an aluminum adjuvant in immunoenhancement effect, little in side effect, wide in source and easy to produce, and a preparation process of the cistanche deserticola polysaccharides is simple, low in cost, high in yield and beneficial to large-scale production.

Description

technical field [0001] The invention relates to an immune adjuvant, in particular to the application of Cistanche deserticola polysaccharide in a vaccine adjuvant, and belongs to the field of biotechnology. Background technique [0002] Herba Cistanche (HerbaCistanche) is a plant in the Dan family. It is a parasitic herb that grows in a desert environment. It is mainly produced in Xinjiang, Gansu, Inner Mongolia, Ningxia, etc. in my country and in the deserts of Central Asia and West Asia. It has the reputation of "Ginseng" in the desert. , Clinical and folk applications are widely used, and "Chinese Pharmacopoeia" records desert cistanche and tube flower cistanche as medicinal cistanche. Xinjiang is the main producing area of ​​Cistanche, mainly producing desert Cistanche and tube flower Cistanche. In the past ten years, researchers have carried out in-depth and meticulous research on its chemical composition and pharmacological effects, and found that Cistanche has various...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61P37/04
Inventor 张爱莲吴道澄杨秀梅赵兵巴雪丽
Owner XINJIANG UNIVERSITY
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