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Special-purpose kit for preparing human dendritic cell vaccines

A technology for preparing dendritic cells and vaccines, which is applied in the field of special kits for the preparation of human dendritic cell vaccines, to achieve the effects of consolidating therapeutic effects, strong selectivity, and improving quality of life

Inactive Publication Date: 2014-03-19
SHENZHEN HORNETCORN BIOTECH
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  • Claims
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Problems solved by technology

[0003] At present, there are many routine methods for DC expansion, such as classic GM-CSF and IL-4 to induce DC differentiation, and then use TNF-α or a combination of cytokines IL-1β, IL-6, TNF-α, PGE-2, etc. Or combined with polyinosinic acid (polycytidylic acid, poly-I: C), bacterial lipopolysaccharide (bacterial lipopolysaccharide, LPS), etc. to promote its maturation, but the above-mentioned DC maturation-promoting agents can only make mature DC secrete IL- 12, and IL-12 is a key molecule for DC to promote the differentiation of T cells to Th1 immune response

Method used

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  • Special-purpose kit for preparing human dendritic cell vaccines
  • Special-purpose kit for preparing human dendritic cell vaccines

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Embodiment 1

[0018] Step 1: further separate and obtain mononuclear cells from the isolated PBMCs. Include the following steps:

[0019] 1. Collect 50ml of peripheral venous blood from the patient, and obtain mononuclear cells by density gradient centrifugation of Ficoll-Diatrizoate Glucosamine. The specific steps are: 1500 rpm, centrifuge for 10 minutes, absorb the upper plasma layer, inactivate at 56°C for 30 minutes and centrifuge for later use, dilute the precipitated blood cells with normal saline, human lymphocyte separation medium and diluted blood at a ratio of 1:2 The proportion of the centrifuge tube was added to the centrifuge tube, 2000 rpm, centrifuged for 20 minutes, carefully sucked the buffy coat layer, washed twice with normal saline, the rotation speed was 1600 rpm, 1300 rpm, and centrifuged for 7 minutes to obtain the peripheral Blood mononuclear cells.

[0020] 2. Resuspend the above isolated PBMCs in the mononuclear cell isolation and acquisition medium, and adjust t...

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Abstract

The invention belongs to the technical field of cellular immunology and specifically discloses a special-purpose kit for preparing human dendritic cell vaccines capable of largely secreting IL(Interleukin)-12. The kit consists of a monocyte separating-obtaining culture medium, a culture medium for promoting DC (Dendritic Cell) to induce and differentiate, an agent for promoting the DC to mature and a tumor antigen. The DC prepared by the special-purpose kit disclosed by the invention is mainly applied to treating cancer patients or preventing cancer high-risk population. The special-purpose kit disclosed by the invention has the advantage that the prepared DC can secrete a great deal of IL-12, so that T cells are promoted to differentiate towards Th1 type immune response direction; and meanwhile, the special-purpose kit has the advantages of being simple in preparation process, low in cost, easy for large-scale production, and the like.

Description

technical field [0001] The invention relates to a special kit for preparing human dendritic cell vaccine, in particular to a special kit for dendritic cell vaccine capable of secreting IL-12 in large quantities. Background technique [0002] Dendritic cell (DC) is the most powerful antigen-presenting cell (APC) known so far, and its biggest feature is that it can stimulate naive T cells ( T cell) activation and proliferation are the enablers of specific immune responses. Therefore, DCs have broad application prospects in tumor immune cell therapy. However, the proportion of DC in human peripheral blood is very low, and the function of DC in tumor patients is mostly in a suppressed state. Therefore, how to obtain a sufficient number of DCs capable of inducing T cells to differentiate into cytotoxic T lymphocytes (cytotoxic T lymphocytes, CTL) in the direction of Th1 immune response has become the key to clinical application. [0003] At present, there are many routine met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
Inventor 马飞黄浩申珊珊
Owner SHENZHEN HORNETCORN BIOTECH
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