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Enteromorpha prolifera oligosaccharide with auxiliary blood sugar decreasing function and preparation method thereof

A technology of enteromorpha oligosaccharides and hypoglycemia, which is applied in the field of medicine to achieve the effects of convenient operation, reduced enzyme dosage and high efficiency

Active Publication Date: 2016-05-11
福州海晟特膳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, no other research reports or patents have been found on the preparation of Chlorella Enteromorpha oligosaccharides and its hypoglycemic activity by microwave-assisted step-by-step enzymatic hydrolysis

Method used

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  • Enteromorpha prolifera oligosaccharide with auxiliary blood sugar decreasing function and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] (1) Dried and crushed Enteromorpha, added 60% ethanol to the dry powder of Enteromorpha and refluxed for extraction for 1 hour, the ratio of dry powder weight to ethanol volume (g / mL) was 1:20, recovered and removed the ethanol for recycling after reflux, and dried Dry to obtain dry defatted Enteromorpha powder;

[0023] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha to distilled water volume (g / mL) is 1:25, 480W microwave-assisted extraction at an extraction temperature of 60-70°C for 2 minutes, After the extraction is completed, filter, repeat the above steps for 2 extractions of the filter residue, mix the obtained filtrate and concentrate, add 3 times the volume of 95% ethanol, settling at 4°C for 8 hours, collect the precipitate by centrifugation, and vacuum freeze-dry After crushing, the crude Enteromorpha sulfated polysaccharide is obtained.

[0024] (3) Add distilled water to the crude s...

Embodiment 2

[0036] (1) Dried and crushed Enteromorpha, added 80% ethanol to the dry powder of Enteromorpha and refluxed for 2 hours, the ratio of dry powder weight to ethanol volume (g / mL) was 1:30, recovered and removed ethanol for recycling after reflux, and dried Dry to obtain dry defatted Enteromorpha powder;

[0037] (2) Add distilled water to the degreased Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha to distilled water volume (g / mL) is 1:30, 600W microwave-assisted extraction at an extraction temperature of 70°C for 3 minutes, and the extraction is complete Afterwards, filter, repeat the above steps for 3 extractions of the filter residue, mix the obtained filtrate and concentrate, add 4 times the volume of 95% ethanol, let it settle at 10°C for 12 hours, collect the precipitate by centrifugation, vacuum freeze-dry and crush , to get crude Enteromorpha sulfated polysaccharide.

[0038] (3) Add distilled water to the crude sulfated polysaccharide ...

Embodiment 3

[0050] (1) Dried Enteromorpha and pulverized it, added 50% ethanol to the dry powder of Enteromorpha and refluxed it for 1.5 hours. The ratio of dry powder weight to ethanol volume (g / mL) was 1:25. Dry to obtain dry defatted Enteromorpha powder;

[0051] (2) Add distilled water to the defatted Enteromorpha powder prepared in step (1), wherein the mass ratio of Enteromorpha to distilled water volume (g / mL) is 1:28, 520W microwave-assisted extraction at an extraction temperature of 65°C for 2 minutes, and the extraction is complete Afterwards, filter, repeat the above steps to extract the filter residue twice, mix the obtained filtrate and concentrate, add 4 times the volume of 95% ethanol, settling at 8°C for 10 hours, centrifuge to collect the precipitate, vacuum freeze-dry and crush , to get crude Enteromorpha sulfated polysaccharide.

[0052] (3) Add distilled water to the crude sulfated polysaccharide of Enteromorpha enteromorpha obtained in step (2), wherein the mass rati...

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Abstract

The invention discloses an enteromorpha prolifera oligosaccharide with an auxiliary blood sugar decreasing function and a preparation method of the enteromorpha prolifera oligosaccharide. The preparation method is characterized by comprising the steps of extracting enteromorpha prolifera sulphated polysaccharide by assisting of using microwaves; degreasing, removing protein and pigments; adding polygalacturonase, alpha-L-rhamnosidase and xylanase in sequence to perform enzymolysis; after enzyme denaturation, removing enzymes and undegraded polysaccharide by using ethanol; enabling a centrifugal supernatant to pass through a molecular sieve to obtain a retentate; freezing and drying the retentate to obtain the enteromorpha prolifer aactive oligosaccharide. Verified by pharmacodynamics of animals, enteromorpha prolifera oligosaccharide can remarkably decrease a blood sugar value of a high blood sugar type mouse. Non-specific commercial enzymes are used, the preparation method disclosed by the invention is simple in process route, highly efficient, and environment friendly; the preparation quantity of the oligosaccharide is improved, and the consumption of enzymes and the production cost are reduced; the preparation method is an effective method for preparing the enteromorpha prolifera active oligosaccharide.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of Enteromorpha oligosaccharides with auxiliary hypoglycemic effect. Background technique [0002] Diabetes mellitus is a systemic metabolic disorder characterized by hyperglycemia caused by defects in insulin secretion and / or its biological function, and its incidence is increasing year by year. Enteromorpha polysaccharides belong to green algae polysaccharides, mostly water-soluble sulfated polysaccharides, mainly glucuronic acid-xylose-rhamnose polymers, which have the effects of lowering cholesterol, lowering blood sugar and regulating blood lipids. Compared with polysaccharides, oligosaccharides have the advantages of being easily soluble in water, non-antigenic, and have a weaker accumulation effect in the host. At present, the research is mainly focused on the polysaccharides of Enteromorpha polysaccharides, and there is no report on the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 赵超刘斌李秋哲吴一晶杨成凤陈玉青
Owner 福州海晟特膳生物科技有限公司
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