AFG2 toxin production fermentation method of aspergillus parasiticus

A technology of Aspergillus parasiticus and a fermentation method, applied in the field of fermentation engineering, can solve the problems of low AFG2 concentration, long time consumption, unevenness and the like, and achieve the effects of low production cost, long time consumption and high spore activity

Active Publication Date: 2018-01-09
沭阳康源泰博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an AFG2 toxin-producing fermentation method of Aspergillus parasiticus, which is used to solve the problems of low, uneven and time-consuming AFG2 concentration in grains naturally infected with molds

Method used

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  • AFG2 toxin production fermentation method of aspergillus parasiticus
  • AFG2 toxin production fermentation method of aspergillus parasiticus
  • AFG2 toxin production fermentation method of aspergillus parasiticus

Examples

Experimental program
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Effect test

Embodiment 1-4

[0024] The preparation of embodiment 1-4 enrichment culture medium

[0025] A bacterium enrichment medium for Aspergillus parasiticus strains, after mixing the components shown in Table 1, adjust the pH value of the bacterium enrichment medium to 5.5-7.0 with hydrochloric acid and sodium hydroxide solution.

[0026] Table 1

[0027]

[0028]

Embodiment 5-8

[0029] The preparation of embodiment 5-8 compound mineral salt

[0030] Take sodium nitrate, magnesium sulfate, potassium chloride, dipotassium hydrogen phosphate, zinc sulfate, manganese sulfate and ferrous sulfate pentahydrate according to the ratio in Table 2, mix the above mineral salts evenly, and pulverize them through an 80-mesh sieve , mixed again for later use, to obtain the compound mineral salts described in the enrichment medium and solid fermentation medium.

[0031] Table 2

[0032]

[0033] Now take the compound mineral salt formula of embodiment 6 and the bacterium enrichment medium formula of embodiment 2 as examples, prepare 100g compound mineral salt and 1L bacterium enrichment medium.

[0034] Composite mineral salt (100g): take by weighing 44.0g sodium nitrate, 14.0g magnesium sulfate, 12.0g potassium chloride, 20.0g dipotassium hydrogen phosphate, 5.7g zinc sulfate, 4.0g manganese sulfate, 0.3g pentahydrate with analytical balance respectively Mix fe...

Embodiment 9-12

[0039] The preparation of embodiment 9-12 Aspergillus parasitica solid fermentation medium

[0040] According to the formula in Table 3, prepare the required solid fermentation medium.

[0041] table 3

[0042]

[0043] Raw material preparation: crush the indica rice and cottonseed meal, and it is required to pass through a 20-mesh standard sampling sieve, but not through a 40-mesh standard sampling sieve, that is, the particle size is between 20-40 mesh.

[0044] Now take the compound mineral salt formula of embodiment 6 and the component of embodiment 10 as example, prepare 100g compound mineral salt and 1L bacteria enrichment medium.

[0045] Composite mineral salt (100g): take by weighing 44.0g sodium nitrate, 14.0g magnesium sulfate, 12.0g potassium chloride, 20.0g dipotassium hydrogen phosphate, 5.7g zinc sulfate, 4.0g manganese sulfate, 0.3g pentahydrate with analytical balance respectively Mix ferrous sulfate in a beaker evenly, then put all the mixed mineral salt...

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Abstract

The invention belongs to the technical field of fermentation engineering, and specifically relates to an AFG2 toxin production fermentation method of aspergillus parasiticus. The method comprises: a,performing reactivation propagation to aspergillus parasiticus spores by adopting an enrichment medium; and b, performing solid fermentation culture through adoption of a solid fermentation medium, wherein each of the enrichment medium and the solid fermentation medium contains 3-5% of yeast extract. The method is used for solving the problem that grain naturally infected with mould is low in AFG2concentration and nonuniform in AFG2 and the culture time is long.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to an AFG2 toxin-producing fermentation method of Aspergillus parasiticus. Background technique [0002] Aflatoxins (AFs) are secondary metabolites produced by some toxin-producing fungi such as Aspergillus parasiticus, Aspergillus flavus and Aspergillus jifeng, which have triple effects of carcinogenicity, teratogenicity and mutagenesis. According to literature reports, more than 20 types of AFs have been identified, the most common of which are aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG2), and aflatoxin G2. (AFG2). AFs can contaminate corn, wheat, soybean, cottonseed and other grain and oil-bearing agricultural products, and can also remain in some agricultural by-products such as DDGS, wheat bran, soybean meal and cottonseed meal. When AFs-contaminated agricultural products are used as human food, they will endanger human health. Mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12N1/14C12R1/66
Inventor 熊江林丁斌鹰侯永清刘玉兰邱银生吴灵英
Owner 沭阳康源泰博生物科技有限公司
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