Method for quickly and efficiently identifying aflatoxin producing strain

Abstract

The invention relates to an identification method and particularly relates to a method for quickly and efficiently identifying an aflatoxin producing strain. In gradient dilution screening, the asperigillus flavus and asperillus parasiticus in a sample are directly applied to a selective aspergillin agar base (AFPA) medium; according to the characteristic that the asperigillus flavus and asperillus parasiticus show bright orange color on the APFA medium, the asperigillus flavus and asperillus parasiticus can be separated from other fungi based on once separation culture; and then the type and content are identified with fluorescence. By adopting the method for identification, the process is simple, the identification is quick, and the specificity is very strong.

Description

technical field [0001] The invention relates to an identification method, in particular to a method for quickly and efficiently identifying aflatoxin-producing strains. Background technique [0002] Aflatoxin (AFT) is mainly a secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus, which is highly toxic and carcinogenic, and is the most toxic type of biotoxin found so far in contaminated agricultural products. When grains are not dried in time or stored improperly, they are often easily contaminated by Aspergillus flavus or Aspergillus parasiticus and produce such toxins. At the same time, they exist in soil, animals and plants, and various nuts, especially easily contaminating grain and oil products such as rice, peanuts, corn, soybeans, and wheat. They are the most toxic mycotoxins and are extremely harmful to human health. . In 1993, aflatoxin was classified as a Class 1 carcinogen by the Cancer Research Institute of the World Health Organizatio...

AI Technical Summary

Problems solved by technology

[0003] The problem of aflatoxin pollution is very serious and can cause huge economic losses, and can cause serious harm to the health of humans, livestock and poultry

The focus of scientific research workers at home and abroad is mainly on the mechanism of aflatoxin production, prevention and control technology, and efficient and sensitive detection of aflatoxin, and a lot of research work has been done, but they have not really started from the toxin production of strains. Effective prevention and control, however, the current isolation methods for aflatoxin-producing bacteria are cumbersome, heavy workload, time-consuming, low isolation efficiency, and difficult to isolate toxin-producing strains in complex matrices such as soil

Moreover, the commonly used method is to first cultivate the Aspergillus flavus strain, then extract the toxin, and finally detect the toxin content, which is very time-consuming and laborious.

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