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Classification and identification upon 12 fungi by using Fourier transform infrared spectroscopic technology

A technology of fungi and Fusarium solani, applied in the measurement of color/spectral characteristics, preparation of test samples, etc., can solve the problem that the spectral technology is not systematic, mature, or standardized, and the FT-IT spectral information sharing database of 13 fungi is open And other issues

Inactive Publication Date: 2013-07-24
威海出入境检验检疫局检验检疫技术中心
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Problems solved by technology

However, the application of spectral technology in the classification and identification of microorganisms is not yet systematic and mature enough, and it is not yet standardized enough. It needs to be unified and form a standard method
At present, there is no standardized and unified FT-IR classification and identification method for the above-mentioned 12 fungi at home and abroad, and there is no disclosure of the FT-IT spectral information sharing database for the 13 fungi. This invention can make up for the above defects and establish 12 fungi Standardized and unified FT-IR classification and identification method

Method used

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  • Classification and identification upon 12 fungi by using Fourier transform infrared spectroscopic technology
  • Classification and identification upon 12 fungi by using Fourier transform infrared spectroscopic technology

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Embodiment

[0021] Preparation of test sample: Divide sterile chili powder into 12 parts, each 500g;

[0022] Bacterial suspension preparation: Scrape off the cultivated mycelium with a small iron spoon that has sterilized the bacteria, put it into a 1.5mL centrifuge tube filled with 1mL sterile saline, and blow and suck several times;

[0023] Addition: Add F.graminearum, A.terricola, P.citreonigrum, A.flavus, A.parasiticus, P.aurantiogriseum, T.pseudokoningii, A.humicola, P.viridicatum, A.funigatus, A. .Uersicolor, F.solani standard strain solution 10mL each, stir and mix, freeze for later use.

[0024] Test the samples for testing as follows:

[0025] Including purified water, 0.9% normal saline, absolute ethanol, potato dextrose agar (PDA), etc.;

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Abstract

The invention belongs to a classification and identification method upon 12 common fungi, and specifically relates to a method for carrying out classification and identification upon 12 common fungi such as F.graminearum, A.terricola, P.citreonigrum, A.flavus, A.parasiticus, P.aurantiogriseum, T.pseudokoningii, A.humicola, P.viridicatum, A.funigatus, A.uersicolor, and F.solani by using a Fourier transform infrared spectroscopic (FTIR) technology combined with chemometric operation (hierarchical cluster analysis). The method comprises the steps of the establishment of a FTIR spectra information database of the 12 fungi, fungi culturing, ZnSe window manufacturing, spectral acquisition and preprocessing, and result determination by hierarchical cluster analysis. The method has the advantages of high resolution, high speed, simple operation, and low cost.

Description

technical field [0001] The present invention belongs to the rapid classification identification method to 12 kinds of common fungi, specifically uses Fourier transform infrared spectroscopy (Fourier transform infrared spectroscopy, FT-IR) technique to Fusarium graminearum (Fusarium graminearum, F. graminearum), habitat Aspergillus terricola (A.terricola), Penicillium citreonigrum (P.citreonigrum), Aspergillus flavuus (A.flavus), Aspergillus parasiticus (A.parasiticus), Penicillium citreonigrum (Penicillium aurantiogriseum, P.aurantiogriseum), Trichoderma pseudokoningii, T.pseudokoningii, Alternaria humicola, A.humicola, Penicillium viridicatum, P.viridicatum, smoke Aspergillus funigatus (A. funigatus), Aspergillus versicolor (A. uersicolor), Fusarium solani (Fusarium solani, F. solani) were rapidly classified and identified. Background technique [0002] Fungi, especially phytopathogenic fungi, are various and widely distributed. They are important pathogens that cause dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N1/28
Inventor 杨丽君李兆杰王静徐成钢
Owner 威海出入境检验检疫局检验检疫技术中心
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