A method for producing malic acid by fermenting wheat bran hydrolysis sugar

A technology of wheat bran and wheat bran powder, which is applied in the field of fermentation, can solve the problems of low utilization rate of wheat bran and the like, and achieve the effects of alleviating energy crisis, low sugar loss and low production cost

Inactive Publication Date: 2016-03-16
HEFEI UNIV OF TECH
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for producing malic acid by hydrolyzing sugar from wheat bran to solve the problem of low utilization rate of wheat bran

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A, the preparation of wheat bran hydrolyzate

[0023] Remove impurities from the wheat bran, place it in a hot air drying oven, and dry it at 80°C for several hours. The dried wheat bran is crushed with a plant pulverizer, passed through a 60-mesh sieve, and the wheat bran powder is packed in a sealed bag for subsequent use.

[0024] Take 200g of wheat bran powder, add 2.0L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix well, react at 121°C for 1h, cool, filter, take the filtrate, and add excess Ca(OH) to the filtrate 2 Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add dilute sulfuric acid with a mass concentration of 3% to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant, that is, the hydrolyzed sugar of wheat bran is obtained.

[0025] B. Hydrolyzate detoxification treatment

[0026] Add 200g of activated carbon to the wheat bran hydrolyzate obtain...

Embodiment 2

[0036] A, the preparation of wheat bran hydrolyzate

[0037] Remove impurities from the wheat bran, place it in a hot air drying oven, and dry it at 80°C for several hours. The dried wheat bran is crushed with a plant pulverizer, passed through a 60-mesh sieve, and the wheat bran powder is packed in a sealed bag for subsequent use.

[0038] Take 15kg of wheat bran powder, add 150L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix well, react at 121°C for 1h, cool, filter, take the filtrate, and add excess Ca(OH) to the filtrate 2Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add dilute sulfuric acid with a mass concentration of 3% to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant, that is, the hydrolyzed sugar of wheat bran is obtained.

[0039] B. Hydrolyzate detoxification treatment

[0040] Add 15kg of activated carbon to the wheat bran hydrolyzate obtaine...

Embodiment 3

[0050] A, the preparation of wheat bran hydrolyzate

[0051] Remove impurities from the wheat bran, place it in a hot air drying oven, and dry it at 80°C for several hours. The dried wheat bran is crushed with a plant pulverizer, passed through a 60-mesh sieve, and the wheat bran powder is packed in a sealed bag for subsequent use.

[0052] Take 100g of wheat bran powder, add 1.0L of dilute sulfuric acid solution with a mass concentration of 3%, stir and mix well, react at 121°C for 1h, cool, filter, take the filtrate, and add excess Ca(OH) to the filtrate 2 Adjust the pH to 10-11, centrifuge at 8000r / min for 10min, take the supernatant, add dilute sulfuric acid with a mass concentration of 3% to the supernatant to adjust the pH to neutral, centrifuge at 8000r / min for 10min, take The supernatant, that is, the hydrolyzed sugar of wheat bran is obtained.

[0053] B. Hydrolyzate detoxification treatment

[0054] Add 100g of activated carbon to the wheat bran hydrolyzate obtain...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for producing malic acid by using fermentation of wheat bran hydrolysate. The method comprises the following steps: preparing wheat bran hydrolysate by an acid hydrolysis method, and then carrying out detoxification treatment on a fermentation inhibitor contained in the hydrolysis liquid glucose by using activated carbon; and selecting aspergillus parasiticus CICC40365 with good adaptability, and matching with the optimized fermentation nutrients to mix and ferment, so as to obtain malic acid fermentation liquor. The method has advantages of being high in wheat bran hydrolysis rate, fast in reaction speed, simple in process and low in cost, the hydrolysis liquid glucose is low in loss in the fermentation process by good detoxification treatment, and the adopted aspergillus has good adaptability, and is high in conversion rate of the malic acid. In addition, the method is free of poison and harm in the production process, environment-friendly and energy-saving, applicable to industrial large-scale production, and significant in economic benefits may be achieved, energy crisis in China and in the world can be effectively relieved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing malic acid by fermenting wheat bran hydrolysis sugar. Background technique [0002] L-malic acid (L-malic acid), also known as hydroxysuccinic acid, is a saturated dibasic acid. Appearance is white crystal or crystalline powder, has strong hygroscopicity, easily soluble in water and ethanol, has a special sour taste, a natural organic acid widely present in vegetables and fruits, its content is second only to citric acid, and Has a special flavor. In addition, L-malic acid is also an important intermediate product in human metabolism, which is easily absorbed by the human body. Therefore, it is widely used in food, cosmetics, medicine and other fields as an excellent sour agent and fresh-keeping agent. [0003] Wheat is one of the main food crops in my country, and its annual output has exceeded 100 million tons. Wheat bran is the main ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/46C12R1/66
Inventor 李兴江吴悦潘丽军姜绍通李硕刘亚孙婷
Owner HEFEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap