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Antagonistic bacterium for controlling radix rehmannia root rot and application of antagonistic bacterium

An antibacterial and root rot technology, applied in bacteria, fungicides, microorganism-based methods, etc., can solve problems such as environmental pollution, increase production input, soil-borne diseases, etc., and achieve the effect of long history of drug use and significant clinical efficacy.

Inactive Publication Date: 2015-09-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuous cropping of Rehmannia rhizome not only caused a sharp decline in yield and quality, but also caused serious soil-borne diseases. What is even more worrying is that when the causes and mechanism of continuous cropping obstacles are not yet clear, most farmers try to increase fertilizers and abuse pesticides. To maintain production, but the effect is often not good, not only increase production input, but also lead to a series of problems such as environmental pollution, excessive pesticide residues in Chinese medicinal materials, and degradation of farmland ecosystem functions, so that the cultivation and production of traditional Chinese medicine resources into a vicious circle

Method used

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  • Antagonistic bacterium for controlling radix rehmannia root rot and application of antagonistic bacterium
  • Antagonistic bacterium for controlling radix rehmannia root rot and application of antagonistic bacterium
  • Antagonistic bacterium for controlling radix rehmannia root rot and application of antagonistic bacterium

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 antagonistic bacteria Pseudomonas aeruginosa ( Pseudomonas aeruginosa LK-12) screening and identification

[0022] 1. Rehmannia-specific Fusarium oxysporum antagonistic bacteria Pseudomonas aeruginosa Screening for LK-12

[0023] Antagonistic bacteria Pseudomonas aeruginosa ( Pseudomonas aeruginosa LK-12) was screened from the rhizosphere soil of continuous cropping Rehmannia glutinosa.

[0024] 1.1 Preparation of Pseudomonas selective medium

[0025] Pseudomonas selective isolation agar (PSIA) preparation method is as follows: Weigh 20 g of soybean-casein digest agar (soybean casein digest agar, SCD) (BD, USA), add 495.5 mL Heat and stir in double-distilled water, fully dissolve, then add 1 mL of 0.1% (wt / vol) crystal violet stock solution, sterilize at 121°C for 15 minutes at high temperature and high pressure, cool to about 50°C, add to the medium 3.5 mL of 5% (wt / vol) furanidine stock solution was mixed thoroughly, poured quickly, cooled ...

Embodiment 2

[0037] Example 2 Bacteriostatic effect detection of antagonistic bacteria and antagonistic substances

[0038] (1) Confrontation culture of antagonistic bacteria

[0039] Prepare PSA or PDA medium, after high temperature and high pressure sterilization at 121°C, quickly invert the plate, cool and solidify to make PSA or PDA plate, draw a cross at the bottom of the culture dish, and inoculate the activated culture at a distance of 2.5 cm from the center of the circle. Pseudomonas was placed in a 28°C constant temperature incubator and cultured in the dark for 48 hours, and the pathogenic fungi were inoculated in the center of the petri dish, and placed in a 28°C constant temperature incubator for several days in the dark, and the inhibition was observed in real time. Bacteria formation. After culturing for 5 days, it was found that Pseudomonas aeruginosa ( Pseudomonas aeruginosa LK-12) can effectively antagonize Rehmannia-specific Fusarium oxysporum, Aspergillus flavus, and...

Embodiment 3

[0043] Example 3 Evaluation of the effect of antagonistic bacteria on preventing and controlling Fusarium oxysporum against Rehmannia glutinosa tissue culture

[0044] MS medium (MS + 0.2 mg / L 6-BA + 0.2 mg / L IBA + 30 g / L sucrose + 7 g / L agar) was prepared for rehmannia root tissue culture seedlings, and 30 mL of medium was added to each tissue culture bottle. High temperature and high pressure sterilization, after cooling and solidification, scald a small groove on the surface of the medium with tweezers, inoculate 2 strains of Rehmannia glutinosa seedlings on one side of the small groove, place them in a constant temperature tissue culture room at 25°C for 45 days, and then place them in the small groove. Add 300 μL of Pseudomonas LK-12 bacterial solution cultivated overnight in the medium, and add the same amount of LB medium to the control group instead, and inoculate the Rehmannia-specialized tip Fusarium oxysporum, during which the Pseudomonas LK-12 bacterial solution wa...

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Abstract

The invention provides an antagonistic bacterium for controlling radix rehmannia root rot. The antagonistic bacterium is named as pseudomonas aeruginosa LK-12 (the Preservation No.: CGMCC 10966) after authentication. The pseudomonas aeruginosa LK-12 disclosed by the invention is obtained by screening from continuous cropping rhizosphere soil of radix rehmannia root through an agar disk diffusion method. Fusarium oxysporum and aspergillus flavus pathogenic bacteria separated from lesion parts of the radix rehmannia root have a high antagonistic effect; in the meanwhile, fusarium oxysporum, fusarium moniliforme, talaromyces sp. and other pathogenic bacteria separated from lesion parts of radix pseudostellariae also have a remarkable restriction effect and can be used for effectively controlling root rot diseases of medicinal plants comprising the radix rehmannia root to provide a strain for overcoming or relieving replant diseases of the medicinal plants. Therefore, the antagonistic bacterium is a potential biological control strain which is good in biological control effect, free of pollution as well as safe and ecological, and is wide in development and application prospects.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium for preventing and treating rehmannia root rot, which can be used to overcome or alleviate the problem of continuous cropping rehmannia loess-borne diseases, and belongs to the technical field of microorganisms and biological control. Background technique [0002] The problem of continuous cropping obstacles has been known for a long time. Many food crops (such as wheat, potatoes), economic crops (such as tobacco, cotton), oil crops (such as soybeans, peanuts), vegetables (such as cucumbers, tomatoes), horticultural crops (such as Watermelon, strawberry), medicinal plants (such as ginseng, rehmannia glutinosa, Panax notoginseng) and plantations (such as poplar, fir) all have different degrees of continuous cropping obstacles. However, the problem of continuous cropping obstacles that has plagued my country's agricultural production for a long time is particularly se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/385
CPCC12N1/205C12R2001/385
Inventor 吴林坤王娟英林文雄吴红淼陈军
Owner FUJIAN AGRI & FORESTRY UNIV
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