Biosensor electrode for detecting aspergillus flavus toxin B1 and method for making same

An aflatoxin and biosensor technology, applied in the field of biosensors, can solve the problems of time-consuming, high technical level requirements, and cumbersome sample processing.

Inactive Publication Date: 2008-07-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TLC method is relatively simple and does not require expensive instruments and equipment, but the pretreatment of the sample is cumbersome, time-consuming, and has poor accuracy, and the use of a large amount of toxic organic solvents is harmful to the experimenters
[0004] The HPLC method requires high equipment costs, high technical level requirements, sample processing is still cumbersome, and the accuracy of the experimental results is easily affected by reagent differences, so it is not suitable for rapid detection; immunochemistry (enzyme-linked immunosorbent assay, including enzyme-linked immunosorbent assay, Radioimmunoassay, affinity chromatography): (1) Enzyme-linked immunosorbent assay, strong specificity, high sensitivity, low cost, suitable for batch

Method used

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  • Biosensor electrode for detecting aspergillus flavus toxin B1 and method for making same
  • Biosensor electrode for detecting aspergillus flavus toxin B1 and method for making same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 is used for detecting aflatoxin B 1 biosensor electrodes

[0059] The present invention is used to detect aflatoxin B 1 The biosensor electrode comprises a base layer and a reaction layer formed on the base layer, the reaction layer includes an electron mediator, a sol-gel film and aflatoxin oxidase, the electron mediator is fixed on the base layer, and the sol-gel Glue embeds aflatoxin oxidase on the electron mediator modified substrate to form a film for the detection of aflatoxin B 1 enzyme-modified electrodes.

[0060] In this example, the electron mediator used is composed of multi-walled carbon nanotubes activated in an acid oxidation environment, specifically, carboxylated multi-walled carbon nanotubes obtained by nitration and oxidation activation treatment, which pass through the sol-gel Embedding and immobilizing aflatoxin oxidase.

Embodiment 2

[0061] Embodiment 2 is used for detecting aflatoxin B 1 Preparation of Biosensor Electrodes

[0062] (1) Preparation of materials

[0063] (1) Working electrode: Platinum electrode was selected and purchased from Tianjin Lanli Technology Company.

[0064] (2) Multi-walled carbon nanotubes (MWNTs): purchased from Shenzhen Nanoport.

[0065] (3) TEOS (tetraethyl orthosilicate, tetraethyl orthosilicate) was purchased from Sigma Company.

[0066] (2), preparation of carboxylated multi-walled carbon nanotube solution

[0067] Reflux the multi-walled carbon nanotubes (MWNT) with aqua regia for 8-10 hours, centrifuge at 10,000 g for 15 minutes, discard the supernatant, wash the precipitate with double distilled water, centrifuge at 10,000 g for 15 minutes, and wash the precipitate. The precipitate is dried in an oven to obtain carboxylated multi-walled carbon nanotubes.

[0068] Weigh 1 mg of the above-mentioned carboxylated MWNT and dissolve it in 10 ml of pure water containing...

Embodiment 3

[0077] Embodiment 3 is used to detect the experimental analysis of the biosensor electrode of aflatoxin B1

[0078] The aflatoxin oxidase-modified platinum electrode described in the present invention is used as a working electrode to form an electrode system together with a common counter electrode and an optional reference electrode.

[0079] In this embodiment, the Ag / AgCl electrode is selected as the reference electrode, the Pt wire electrode is used as the counter electrode to form a three-electrode system, and the phosphate buffer is used as the electrolyte.

[0080] (1) Processing of samples:

[0081] Solid sample: 100g sample is pulverized with a pulverizer, fully dissolved and shaken with methanol:water=45:55 volume ratio, extracted 3 times with an equal volume of chloroform, passed through a funnel containing anhydrous sodium sulfate, blown nitrogen at 65°C and evaporated to dryness .

[0082] Liquid sample: 100ml sample was extracted 3 times with 0.5:1 (volume:vol...

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Abstract

The invention discloses a biosensor electrode for detecting aflatoxin B1 and a preparation method thereof. The biosensor electrode comprises a substrate layer and a reaction layer provided on the substrate layer. The reaction layer includes an electronic mediator, a sol-gel film and aflatoxin oxidase, wherein the electronic mediator is fixed on the substrate layer, and the sol-gel film embeds the aflatoxin oxidase on the substrate modified by the electronic mediator to form a film, thus obtaining aflatoxin oxidase modified electrode. The inventive biosensor electrode has the advantages of fast response, high signal sensitivity up to 1.5 ppm to 0.125 ppb, high specificity, convenient usage, quantitative detection, long service life and good stability.

Description

technical field [0001] The invention belongs to the field of biosensors, and relates to a method for detecting aflatoxin B 1 A biosensor electrode and a preparation method thereof. Background technique [0002] Aflatoxin B 1 (AF B 1 ) is one of the currently known strong carcinogens, which often contaminate animal feed and human food. In 1988, the International Agency for Research on Cancer (IARC) listed aflatoxin AFB1 as a human carcinogen. Feeding livestock with contaminated feed can lead to contamination of meat, milk and their products. If people eat contaminated food, it will cause acute poisoning, liver necrosis and hemorrhage, and chronic poisoning can cause liver cancer and lung cancer. At the same time, feeding poultry and livestock with contaminated feed will reduce the productivity of poultry and livestock, slow down the weight gain, and cause major economic losses. All countries have strict limits on aflatoxins in food and feed. [0003] At present, aflatox...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 刘大岭李世川姚冬生谢春芳
Owner JINAN UNIVERSITY
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