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Method for accurately and rapidly checking aspergillus flavus toxin

An aflatoxin, fast technology, used in biological testing, material inspection products, color/spectral property measurement, etc., can solve problems such as high price and short analysis time, and achieve high accuracy, good repeatability, and enhanced specificity. Effect

Inactive Publication Date: 2008-10-15
上海纤检仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the high specificity and short analysis time of this method, it has been widely valued. Later, someone developed a "pill box" using ELISA. One box of reagents can measure multiple test samples. available, but more expensive

Method used

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  • Method for accurately and rapidly checking aspergillus flavus toxin
  • Method for accurately and rapidly checking aspergillus flavus toxin
  • Method for accurately and rapidly checking aspergillus flavus toxin

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Embodiment

[0076] Such as figure 1 As shown, it is a flow chart of a method for accurate and rapid detection of aflatoxin, and a method for accurate and rapid detection of aflatoxin is:

[0077] 1. Standard sample preparation

[0078] The first step, preparation of aflatoxin AFB 1 Antibody coated reaction plate:

[0079] anti-aflatoxin AFB 1 Polyclonal antibody with 70mmol / L, Na 2 CO 3 -NaHCO 3 PH9.6 buffer diluted to 4μg / ml coating solution, added to the 48 or 96-well test tube of the reaction plate, add 100μL to each well, put in a 4°C refrigerator overnight, discard the coating solution and wash 3 times, each well Add 200 μL of 100 mmol / L, pH7.4 phosphate PBS buffer solution containing 1% bovine serum albumin BSA to seal, put at 37 ° C for 3 hours, discard the blocking solution, vacuum-pack the coated plate, and store it in a freezer at -20 ° C;

[0080] The second step is to prepare the enzyme-labeled antigen:

[0081] Take 1mg aflatoxin B 1 ——Carboxymethyl oxime AFB 1 -omi...

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Abstract

The invention relates to a method for realizing accurate and quick detection of aflatoxin. The invention is characterized in that the method is as follows: step (1), enzyme-labelled antigen, an aflatoxin AFB1 standard solution, sample diluent and enzyme-labelled antigen diluent are made, and a substrate solution a, a substrate solution b, a stop solution (i.e. a 2mol / L sulphuric acid solution) and extract are made by a cleaning solution; step (2), to-be-measured solution pretreatment, reagent preparation and reaction plate test tube numbering are carried out, and a prepared solution, a to-be-measured sample solution and the stop solution are added in turn; meanwhile, a reaction plate is arranged, and an aflatoxin tester is used to measure the absorbance A value of each hole; then, according to the measured absorbance A value, an equation A=MC+N for regression calculation is automatically established inside the aflatoxin tester to calculate the content of AFB1 in sample. The method has the advantages of simplicity and convenience, quickness, high accuracy and high sensitivity.

Description

technical field [0001] The invention relates to a method for accurately and rapidly detecting aflatoxin, belonging to the technical field of immune enzymes in bioengineering. Background technique [0002] Aflatoxin (abbreviated as AFT) is a mycotoxin, which is a highly toxic secondary metabolite produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Oil crop seeds, fruits, dried fruits, vegetables, condiments, tobacco, jute, milk, dairy products, meat, fish and shrimp, fermented products and feed are all contaminated by AFT. [0003] The physical and chemical properties of AFT are very stable. It will not be destroyed under the high temperature of 200 ℃ and ultraviolet radiation, and it can be transferred from one organism to another through the action of the food chain. 1. Milk toxins can enter the human body with food and cause human poisoning or cancer. According to reports from the World Health Organization and Shanghai Cancer Institute and other relevant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/25
Inventor 潘中华成恒嵩赵晓联葛其德
Owner 上海纤检仪器有限公司
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