Method for rapidly detecting content of aflatoxin in brown rice based on attenuated total reflection Fourier transform infrared spectrum technique

Abstract

The invention provides a method for rapidly detecting the content of aflatoxin in brown rice based on an attenuated total reflection Fourier transform infrared spectrum technique. The method comprises the following steps: preparing a sample: collecting brown rice samples with different aspergillus flavus invaded degrees, crushing the brown rice samples so as to obtain sample powder, and refrigerating for detecting; detecting by spectrum: scanning a spectrum signal of a sample by adopting a Fourier transform infrared spectroscopy, picking partial screened samples, and determining the level of aflatoxin B1, B2, G1 and G2 and the total quantity thereof in the brown rice sample powder; carrying out data preprocessing: preprocessing the original spectrum information of the sample powder to eliminate interference; quantitatively predicting and analyzing: establishing a relevant relational model of the real content level of aflatoxin in the brown rice samples and the prediction level according to the correspondence of the level of the aflatoxin B1, B2, G1 and G2 and the total quantity thereof in the brown rice samples and the corresponding spectrum adsorption value based on a partial least squares regression (PLSR) method; and determining rapidly: outputting the aflatoxin B1, B2, G1 and G2 and the total quantity level thereof based on the spectrum information of the to-be-detected brown rice by utilizing the established model.

Description

technical field [0001] The invention relates to the technical field of grain quality detection, in particular to a rapid detection method for aflatoxin content in brown rice based on attenuated total reflection Fourier transform infrared spectroscopy technology. Background technique [0002] With the development of science, the nutritional value of brown rice has been paid more and more attention by people. After analyzing the embryo and bran of brown rice, the U.S. Food and Drug Administration found that 10g of brown rice contains 3g of protein, 1.2g of vegetable fat, and 2.5g of B vitamins. Moreover, vitamin B in brown rice 1 , B 2 , E, C, D, calcium, iron and cellulose are all higher than polished rice. In addition, brown rice is rich in nutritional and health functional factors, while polished rice does not contain or has a low content. For example, glutathione with antioxidant activity in brown rice is about 3.64mg / 100g, and contains ferulic acid group γ -Oryzanol, ...

AI Technical Summary

Problems solved by technology

However, TLC has the disadvantages of cumbersome sample pretreatment, low sensitivity, poor reproducibility, and low safety; HPLC has the advantages of good accuracy, high sensitivity, and low detection limit, but the sample pretreatment is complicated and requires a wide variety of chemical reagents. Moreover, professional and technical personnel are required, and it is difficult to meet the requirements of rapid and online detection; ELISA has certain requirements for the storage conditions and time of applicable enzymes and antibodies, and is prone to false positive results; the microcolumn method cannot complete the detection of the entire aflatoxin Testing process, only for qualitative testing

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