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Bacillus subtilis strain, preparation method, preparation preparing proess and use

A technology of Bacillus subtilis and Bacillus, applied in botany equipment and methods, mutant preparation, bacteria, etc., can solve the problems of mutagenesis, carcinogenicity, teratogenicity, etc., achieve good thermal stability of substances, and stable and simple fermentation process , the effect of good application prospects

Inactive Publication Date: 2005-03-09
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, long-term use of certain chemical pesticides will easily lead to resistance of diseases and pests; and some chemical pesticide varieties have been proven to be carcinogenic, teratogenic and mutagenic; at the same time, pesticide residues are hidden dangers that restrict the production of agricultural products

Method used

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Embodiment Construction

[0023] 1. Screen and isolate Bacillus subtilis JA, which has a strong inhibitory effect on wheat head blight, from the soil as the original strain.

[0024] 2. The ion implantation experiment of the original strain was carried out on the low-energy ion beam cell modification technology and device. The patent number of the device is 93103361.6. Use N + Pulse irradiation treatment, after 20keV, 2.6×10 15 ~3.9×10 15 ions / cm 2 , different doses of N + Injection, the process is: place the sample on a water-cooled sterile target platform, make JA into a bacterial suspension, take 0.1mL of the bacterial suspension and spread it on a Φ90mm blank plate, and air-dry it in an ultra-clean bench to make a bacterial film for use; with different doses of N + After the irradiation treatment, the bacterial film was eluted with 1mL sterile water and cultured in solid medium for 24hrs to pick a single colony; after ion beam implantation, a ring of cells was picked from the slant of 24h cult...

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Abstract

A Bacillus subtitis JA with strong supperssion to wheat bakanae fungus is prepared by separating it from soil. Its product is prepared through injecting positive N ions for inducing mutant strains, microbial fermenting to obtain fermented liquid, ultrafiltering, and concentrating. It can be used for preventing and treating more than 9 diseases and pests of agricultural crops, such as wheat bakanae, watermelon blight, etc.

Description

technical field [0001] The present invention relates to a strain of microorganisms, more specifically to a strain of Bacillus subtilis. Background technique [0002] In order to deal with pests and diseases, more than 2 million tons of pesticides are produced in the world every year, mainly chemical pesticides. However, long-term use of certain chemical pesticides will easily lead to resistance of diseases and pests; and some chemical pesticide varieties have been proven to be carcinogenic, teratogenic and mutagenic; meanwhile, pesticide residues are a hidden danger that restricts the production of agricultural products. The biggest advantage of biopesticides is that they are safe to humans and animals, non-toxic, do not pollute the environment, and are not easy to develop resistance. They are increasingly becoming the only choice for the development of green agriculture. Microbial pesticides are recognized as "pollution-free pesticides". The control objects are not easy to...

Claims

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Application Information

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IPC IPC(8): A01N63/02C12N1/20C12N15/01
Inventor 刘静王军余增亮
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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