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Trichoderma longibrachiatum strain and application thereof

A technology of Trichoderma longibrachiae and strains, applied in the direction of fungi, enzymes, biochemical equipment and methods, etc., can solve the problems of few research reports, achieve rapid growth and reproduction, high enzyme activity efficiency, and reduce pollution

Inactive Publication Date: 2014-05-21
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are very few research reports on Trichoderma longibranchus as a cellulase producer

Method used

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  • Trichoderma longibrachiatum strain and application thereof
  • Trichoderma longibrachiatum strain and application thereof
  • Trichoderma longibrachiatum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Separation and purification

[0047] 1. The isolated sample was taken from a soil sample in Hainan, and the collected soil sample was separated and purified by a combination of primary screening and secondary screening. Take a small amount of sample and put it in a sterile Erlenmeyer flask, add 10 times the volume of sterile water, and fully disperse it. Take 1mL-5mL (according to the inoculum size of 2%-10%) and add the suspension into a sterile Erlenmeyer flask filled with 50mL enrichment medium, and incubate at 28°C and 150r / min for 1-2d. The samples after enrichment culture were made 10 0 、10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Gradient dilution. Take 0.1mL and spread them on the re-screening medium respectively, and culture them upside down at 28°C for 3-5d. Strains capable of vigorous growth are isolated.

[0048] 2. Transfer and inoculate the obtained pure strains on a slant medium (PDA comprehensive medium), and store at 4°C.

[0049]The preparat...

Embodiment 2

[0060] 1. Spore culture of Trichoderma longibrachiata CD-6

[0061] Sporulation medium: yeast extract powder 10g, glucose 20g, water 1000ml, pH natural, sterilized at 121°C for 20min.

[0062] Use an inoculation shovel to dig out a piece of mycelium with a side length of about 0.5 cm from the slope and inoculate it on a sporulation medium plate, and culture it at 30°C for 2 to 3 days to produce a large number of gray-green spores.

[0063] 2. Production of cellulose degrading enzymes by fermentation

[0064] Wash the spores cultivated in step 1 with sterile water, adjust the spore concentration with sterile water after counting with hemocytometer, then inoculate the spore suspension in 50mL enzyme-producing medium (packed in a 250mL Erlenmeyer flask) to make The final concentration of inoculated spores was 10 4 ~10 6 Within the range of spores / mL culture medium, shake culture at 30°C and 200rpm for 6-7 days. The enzyme activity of the fermentation broth was measured every ...

Embodiment 3

[0078] The formulation of the enzyme-producing medium is the same as in Example 2.

[0079] Inoculate the spore suspension into 200ml enzyme medium by the method and concentration of Example 2, 30 DEG C, 200rpm cultivates 6-7 days, the highest activity of measuring CMCase in the fermented liquid is 50.65IU / ml, and the highest activity of FPA is 105.09 IU / ml, the highest activity of xylanase is 172.62IU / ml.

[0080] The fermentation culture of embodiment 4 Trichoderma longibrachiata CD-6---cellulase production (three)

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Abstract

The invention relates to a trichoderma longibrachiatum strain and an application thereof. A novel trichoderma longibrachiatum strain CD-6 capable of producing a cellulose degrading enzyme is screened out by the invention. The trichoderma longibrachiatum strain CD-6 is convenient for culture and rapid in growth and reproduction, has relatively high production capacity for endoglucanase, exoglucanase and xylanase, and has relatively high enzyme activity.

Description

Technical field: [0001] The present invention relates to a new Trichoderma longibrachiae strain CD-6, and also relates to the application of the Trichoderma longibranchus in producing exoglucanase, endoglucanase and xylanase. Background technique: [0002] Cellulosic matter is the most abundant, renewable carbohydrate resource on earth. [0003] Cellulase is a complex enzyme product that can degrade cellulose into glucose, and convert it into sugar, ethanol and other chemical products needed by human society. Therefore, the development of cellulose degrading enzymes with good activity can effectively develop and utilize cellulose, a huge renewable resource, which is of great significance to solving the energy crisis. [0004] Trichoderma is one of the main producing strains of cellulase industry, with strong viability, high spore production, complete cellulase production system and high enzyme production efficiency, especially Trichoderma reesei has become a cellulase produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/885
Inventor 顿宝庆田谷王智
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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