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A kind of Bacillus velesi and its application in the antagonism of tomato fusarium wilt

A technology of tomato fusarium wilt and bacillus, applied in application, bacteria, fungicides and other directions, can solve problems such as yield reduction, achieve high application value, increase crop yield, and reduce the use of pesticides

Inactive Publication Date: 2011-12-21
新疆万康诚一肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, processed tomatoes are still affected by various diseases during the years of planting, among which tomato fusarium wilt is one of the more serious diseases, and in severe cases, it will cause a large area of ​​production reduction

Method used

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  • A kind of Bacillus velesi and its application in the antagonism of tomato fusarium wilt
  • A kind of Bacillus velesi and its application in the antagonism of tomato fusarium wilt
  • A kind of Bacillus velesi and its application in the antagonism of tomato fusarium wilt

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation, screening and identification of Bacillus velezensis CGMCC No.4992

[0021] (1) Isolation and screening of antagonistic bacteria

[0022] Gradient dilution separation method: Weigh 10g of tomato rhizosphere soil sample and put it into a triangular flask filled with 90mL sterile water and glass beads, shake for 20min and then let it stand for 20-30s, which is 10 -1 diluent, and then use the gradient method to dilute to 10 -2 to 10 -6 diluent. Take 0.1mL of each dilution (10 -3 、10 -4 、10 -5 、10 - 6 ) were spread on the LB medium respectively, cultured at 37°C, and after the isolates grew out, the colonies grown on the plate were streaked, separated and purified, and finally the slant was streaked and stored for later use.

[0023]Plate confrontation method to screen antagonistic bacteria: The plate confrontation method was slightly modified, and the pathogenic bacteria of tomato wilt was activated on the slope of PDA medium. After the hyphae ...

Embodiment 2

[0039] Embodiment two: the growth factor of Bacillus velezensis CGMCC No.4992

[0040] Refer to the following methods, but the specific growth factors should be determined according to the characteristics of Bacillus velezensis CGMCC No.4992 provided by the present invention.

[0041] The strain LYT-5 was inoculated on LB medium containing different concentrations of salt (NaCl), antibiotic (ampicillin) and pH, and cultured in a 30°C incubator for 14 hours. Then the strain LYT-5 was connected to the LB medium, and placed in 4°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, respectively. Cultivate in an incubator. The results are shown in Table 2.

[0042] It can be concluded from Table 3 that strain LYT-5 is the most suitable growth factor.

[0043] Table 2: Effects of temperature, pH, salt, and antibiotics on the growth of strain LYT-5

[0044] temperature(℃)

Embodiment 3

[0045] Embodiment three: the antagonistic ability of Bacillus velezensis (Bacillus velezensis) CGMCC No.4992 fermented liquid to tomato Fusarium wilt

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Abstract

The invention discloses a Bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.4992 and application thereof in tomato blight antagonism bacteria. A farmland soil sample on which tomatoes are planted for years in Xinjiang Fukang is subjected to antagonism bacteria screening to obtain a strain with stronger bacteriostatic activity; the strain is further screened and domesticated and bred; and the obtained strain is subjected to morphological specificity and 16S rRNA (Ribose Nucleic Acid) sequencing and system development analysis. The antagonism of the fermentation broth of the strain has outstanding technical effect on solving the tomato blight antagonism bacteria and has wide application field.

Description

field of invention [0001] The invention relates to the technical field of biological control, in particular, the invention relates to the technical field of a bacillus velei and its application in the antagonism of tomato wilt. Background technique [0002] Tomato Fusarium wilt is a soil-borne disease that seriously endangers the quality and yield of vegetables. Tomatoes are infected by Fusarium wilt from the seedling stage to the adult plant stage. The pathogen survives the winter in the soil and spreads with irrigation water and soil, causing serious losses to tomato production in severe cases. Pesticide-based control measures not only severely damage the soil ecological structure, but also pose great harm to people's health and the environment. Compared with the environmental pollution and pesticide residue problems caused by the application of chemical pesticides, biological control The method has the advantages of safety, economy and high efficiency. This research sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 詹发强杨文琦侯新强侯敏杨蓉崔卫东龙宣杞张慧涛
Owner 新疆万康诚一肥业有限公司
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