Preparation and application of composite bioactive material taking attapulgite to load bacillus

A technology of attapulgite and bacillus, applied in the field of microorganisms, can solve the problems of low effectiveness and harmful residues

Inactive Publication Date: 2016-01-27
COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method that can not only promote plant growth, prevent and control soil-borne diseases, but also alleviate soil pollution, in order to overcome the harmful residues of chemical pesticides and chemical fertilizers in farmland, and the low effectiveness of single materials as fertilizers or soil sewage improvers. A new type of environmentally friendly microbial-mineral composite material that compacts, enhances the organic content of the soil, improves soil moisture and fertilizer retention, degrades heavy metals and harmful substance residues in the soil, and improves the quality of agricultural products.

Method used

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  • Preparation and application of composite bioactive material taking attapulgite to load bacillus
  • Preparation and application of composite bioactive material taking attapulgite to load bacillus
  • Preparation and application of composite bioactive material taking attapulgite to load bacillus

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Embodiment 1

[0043] The present invention consists of the following materials and their percentage distribution ratios: attapulgite 100%, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) 5%, and the number of viable spores of Bacillus amyloliquefaciens per gram is 1.0~2.0×10 11 indivual.

[0044] Preparation process of the present invention:

[0045] Attapulgite acid-modified preparation method:

[0046] (1) Weigh 10 g of purified attapulgite and put it into a 250 mL Erlenmeyer flask, add a modifier of 1 mol / L HCI, and mix the purified attapulgite and HCI at a ratio of 1:10.

[0047] (2) Place the mixed sample on a shaking table at 150 rpm for 2 hours at 25° C. for modification.

[0048] (3), take out the sample and carry out centrifugation in a 4000rpm centrifuge, discard the supernatant, then wash with distilled water and centrifuge.

[0049] (4) After detecting the absence of chloride ions with 0.1 mol / L silver nitrate solution, dry at 105° C. to prepare modified attapulgite. ...

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Abstract

The invention relates to a method for preparing a composite material which takes modified or unmodified attapulgite as a carrier to immobilize bacillus, belonging to the technical fields of microbial fertilizers, biological control of plant diseases, soil remediation and pollution abatement. A microorganism-mineral immobilization technology is realized by means of preparing a proper amount of attapulgite with a proper amount of bacillus viable bacteria preparation according to a certain technology. The composite material prepared according to the method disclosed by the invention has the advantages that the plant growth can be promoted, the soil-borne diseases, such as root rot, blight, soft rot and the like, of various crops can be inhibited, and the effect of preventing threadworm from spawning is achieved; the composite material further has the capabilities of keeping the local moisture of plant root systems, and enhancing the plant resistance to drought, saline and alkaline as well as oxidization; moreover, the composite material can be further used for purifying organic pollutants in water, absorbing soil heavy metals and relieving soil hardening, so that the effects of soil moisture conservation and fertilizer conservation are achieved. The composite material prepared according to the method disclosed by the invention is an environment-friendly composite material, which has the advantages that environment harm and pollution can be prevented through correct use of the composite material, and a wide application prospect is obtained.

Description

[0001] FIELD OF THE INVENTION The present invention is in the field of microorganisms. It mainly relates to the preparation and application of mineral composite materials of attapulgite immobilized Bacillus. Background technique [0002] With people's emphasis on the ecological environment and the increasing demand for green, safe, organic and pollution-free food, environmentally friendly pesticides and fertilizers have increasingly become research hotspots in the development of agricultural production. For more than half a century, although chemical pesticides have played an important role in agricultural production, the long-term irrational use of chemical pesticides has caused many problems. For example, the high toxicity and high residue of chemical pesticides not only cause harm to human and animal health , and cause serious pollution to soil, water, and atmosphere, destroying the ecological balance. At the same time, due to the increasing resistance of germs and pests, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C02F3/34C05G3/80
Inventor 王若愚尚千涵
Owner COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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