Method for increasing natamycin action efficiency

A natamycin and efficiency technology, applied in the field of improving the efficiency of natamycin, can solve the problems of poor water solubility of natamycin, and achieve the effects of poor water solubility, increased water solubility and stability, and improved action efficiency

Inactive Publication Date: 2009-07-29
NAT ENG AN TECH RES CENT FOR PRESERVATION OF AGRI PROD TIANJIN
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for improving the action efficiency of natamycin, solve the problem of poor water solubility

Method used

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  • Method for increasing natamycin action efficiency

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example 1

[0018] Example 1: Prepare 500 mL of γ-cyclodextrin solution with ultra-pure water to make the concentration reach 70 mM, add 1.7 g of natamycin to the γ-cyclodextrin aqueous solution, and sonicate for 5 minutes. Wrap the above suspension in a container with a layer of aluminum foil, place it on a shaker at room temperature for reaction, the shaker rotates at 200rpm, and reach equilibrium in 24 hours to obtain the natamycin / γ-cyclodextrin supramolecular package a homogeneous solution of the compound. A certain amount of this solution was filtered through a 0.45 μm ultrafiltration membrane, and determined by high performance liquid chromatography. The concentration of the final solution was 3.3 g / L.

[0019] Example 2: Prepare 500 mL of β-cyclodextrin solution with ultrapure water to make the concentration reach 16 mM, add 1.1 g of natamycin to the β-cyclodextrin aqueous solution, and sonicate for 5 minutes. Wrap the above suspension in a container with a layer of aluminum foil...

example 3

[0020] Example 3: Prepare 500 mL of hydroxypropyl-β-cyclodextrin solution with ultrapure water to make the concentration reach 70 mM, add 2.5 g of natamycin to the aqueous solution of hydroxypropyl-β-cyclodextrin, and sonicate for 5 minutes. Wrap the above suspension in a container with a layer of aluminum foil paper, place it on a shaker at room temperature for reaction, the shaker rotates at 200rpm, and reach equilibrium in 24 hours to obtain natamycin / hydroxypropyl-β-cyclopaste A homogeneous solution of refined supramolecular clathrates. Take a certain amount of this solution and filter it with a 0.45 μm ultrafiltration membrane, and measure it by high performance liquid chromatography. The concentration of the final solution is 4.8 g / L.

[0021] Example 4: Prepare 500 mL of disaccharide-β-cyclodextrin solution with ultrapure water to make the concentration reach 70 mM, add 1.25 g of natamycin to the aqueous solution of disaccharide-β-cyclodextrin, and sonicate for 5 minute...

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Abstract

The invention relates to a method for improving the natamycin action efficiency. The method comprises the following steps: taking cyclodextrin and derivatives as the supermolecule host compounds to prepare a natamycin/cyclodextrin supermolecule clathrate; and keeping the bacteriostatic activity of natamycin and improving the water solubility and stability of natamycin. The preparation method for the natamycin/cyclodextrin supermolecule clathrate comprises the following steps: preparing an aqueous solution of the cyclodextrin and the derivatives through ultra pure water to reach certain solubility; adding a certain amount of natamycin into the aqueous solution of the cyclodextrin and the derivatives, and carrying out ultrasonic treatment for 5 minutes to improve the complexation efficiency; and covering a layer of aluminum-foil paper on the container of the suspension, placing the container on a shaking table at a room temperature for reaction, and obtaining the homogenous solution of the natamycin/cyclodextrin supermolecule clathrate after reaching the balance. The method has the advantages of improving the solubility and stability of natamycin in the aqueous solution, improving the natamycin bioavailability, solving the problem of poor water solubility of natamycin, and improving the action efficiency of the biological bacteriocide natamycin.

Description

technical field [0001] The invention relates to a method for antisepsis and preservation of antibiotics as natural biological preservatives, in particular to a method for improving the action efficiency of natamycin, increasing the solubility and stability of natamycin in aqueous solution. Background technique [0002] Natamycin is a broad-spectrum antifungal polyene macrolide antibiotic. Natamycin has attracted much attention for its safety, natural and healthy characteristics. As a natural biological preservative, it has the following characteristics: low dose, high efficiency, and does not affect the flavor of food. Compared with other antibacterial agents, natamycin has extremely low toxicity and has no carcinogenic, teratogenic, or mutagenic effects. Natamycin has been approved to be used in many food industries such as dairy products, meat, fruits, and beverages, and most of them are soaked or sprayed in suspension. [0003] The antibacterial mechanism of natamycin li...

Claims

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Application Information

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IPC IPC(8): A01N25/22A01N25/02A01N43/24A01P1/00A23L3/3562
Inventor 黄艳凤张平李江阔
Owner NAT ENG AN TECH RES CENT FOR PRESERVATION OF AGRI PROD TIANJIN
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