Streptomyces lydicus producing natamycin and uses thereof

A technology of streptomyces lidi and natamycin, applied in the field of microorganisms, can solve the problems of ineffectiveness of bacteria and viruses

Active Publication Date: 2008-05-21
中农绿康(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Natamycin is resistant to almost all molds and yeasts, and its antibacterial effect is about 50 times stronger than that of sorbic acid,

Method used

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  • Streptomyces lydicus producing natamycin and uses thereof
  • Streptomyces lydicus producing natamycin and uses thereof
  • Streptomyces lydicus producing natamycin and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, separation and preparation of Streptomyces lydicus (Streptomyces lydicus) A01 CGMCC No.1653

[0038] Collect soil samples from vegetable fields in Miyun County, Beijing, take 10g and add them to a conical flask containing 100ml sterile water and an appropriate amount of glass beads, place on a shaker and vibrate at 100rpm for 20min, take 0.5ml and add them to 4.5ml sterile water, and then dilute for 10 2 、10 3 、10 4 Take 0.1ml of the above soil suspension and spread it evenly on the Gaoshi No. 1 medium plate, and blow it in the ultra-clean workbench until it is slightly dry; each concentration is repeated 3 times, and placed in a 28°C incubator for 5- On the 10th day, a single colony of actinomycetes was picked for dilution, streaking, isolation and purification to obtain a pure culture strain.

[0039] Preliminary screening: The obtained pure culture strains were subjected to shake flask fermentation culture, and the fermentation liquid was filtered and...

Embodiment 2

[0057] Embodiment 2, the bacteriostatic activity assay of Streptomyces lydicus (Streptomyces lydicus) A01 CGMCC No.1653 fermented liquid

[0058] Slant culture: Streptomyces lydicus (Streptomyces lydicus) A01 CGMCC No.1653 was inoculated on Gao's No. 1 slant medium, and cultured at 28°C for 7-10 days until it produced a sufficient amount of spores;

[0059] Seed culture: Scrape 3-5 rings of spores with a sterile platinum ring and inoculate them into a 500ml Erlenmeyer flask containing 100ml of seed medium, place on a temperature-controlled shaker, and at 28°C, with a rotation radius of 13mm and 180rpm Shake culture at constant temperature at a constant speed for 20-24 hours;

[0060] Fermentation culture: Take the above-mentioned seed culture solution and inoculate it into a 500ml Erlenmeyer flask containing 100ml of fermentation medium at an inoculum size of 3% (V / V). Under the condition of 28°C, shake at a constant temperature with a rotation radius of 13mm and a speed of 18...

Embodiment 3

[0062] Embodiment 3, the antibacterial mechanism of Streptomyces lydicus (Streptomyces lydicus) A01 CGMCC No.1653 metabolites

[0063] 1. Crude extraction and preliminary identification of natamycin in fermentation broth

[0064] The fermented broth obtained in Example 2 was pretreated with 3 times the volume of absolute ethanol, and stood at 4°C for 2 hours to precipitate bacteria, solid particles, soluble viscose, nucleic acids, miscellaneous proteins, and intermediate metabolites, etc., above The supernatant was vacuum-filtered with a Buchner funnel with two layers of filter paper, and the filtrate was concentrated under reduced pressure at 45°C by a rotary evaporator.

[0065] The chemical type of the active substance was identified by paper chromatography of the Czech eight-solvent system, and the ultraviolet full-wavelength scanning was carried out at 190nm to 400nm.

[0066] 1. Paper chromatography of the Czech eight-solvent system

[0067] Solvent system is: I. water...

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Abstract

The invention discloses a strain of Streptomyces lydidii and its application. The strain is Streptomyces lydicus A01 CGMCC No.1653. The bacterial strain can produce natamycin and will be widely used in the preparation of inhibitors of natamycin-sensitive bacteria.

Description

technical field [0001] The invention relates to a strain of Streptomyces lydida in the field of microorganisms and its application. Background technique [0002] Natamycin (Natamycin) is a polyene macrolide antifungal antibiotic, also known as natamycin or Pimaricin (Pimaricin), which is a multi-enzyme system encoded by five polyacetyl synthetase genes Synthetic, capable of obligately inhibiting yeasts and moulds, preventing the formation of aflatoxins in filamentous fungi. Its actual dosage is 10 -6 Order of magnitude, with the characteristics of low dosage, high efficiency, and long antibacterial action time, it is an efficient and safe natural biological food preservative and antibacterial additive, which has been widely used in more than 30 countries, including the European Union, most of North America and Eastern European countries, some Middle Eastern countries, and even Switzerland, which attaches great importance to food safety, allow the use of natamycin in bread ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/02C12P17/08C12R1/465
Inventor 刘伟成刘建华裘季燕卢彩鸽刘霆刘德文
Owner 中农绿康(北京)生物技术有限公司
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