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Preparation method of natamycin

A technology of natamycin and fermentation, which is applied in the direction of antifungal agents and fermentation, can solve the problems of complicated natamycin process, high cost, and by-products, so as to promote the industrialization process, reduce production costs, and improve Yield effect

Inactive Publication Date: 2004-07-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention solves the problems that foreign culture medium is used for fermentation production of natamycin, which is difficult to buy in China, and the price is relatively high, and the process of extracting natamycin from fermentation liquid is complex, expensive or has by-products; it provides a Economical and practical, using domestic culture medium, the preparation process of extracting natamycin through controlled conditional fermentation and evaporation to remove methanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The production strain is Streptomyces gilvosporeus, and its original strain was obtained from the American Type Culture Collection Center, and its deposit number is ATCC 13326. High-yielding strains were screened through DES and UV mutagenesis. The production strain was grown on an agar slant for 5 days and stored in a refrigerator at 4°C for 10 days before use. Among them, the liquid used to make the spore suspension is sterile water or fresh seed culture medium. The sterilization conditions are: 121°C, sterilization for 20-30 minutes. The spore concentration was selected as 108 / mL.

[0064] The seed culture medium is composed of carbon source, nitrogen source, inorganic ions and trace elements, namely:

[0065] Ordinary peptone 6g / L

[0066] Yeast powder 6g / L

[0067] Glucose 20g / L

[0068] NaCl 10g / L

[0069] pH 7.0

[0070] The prepared spore suspension is added to the above-mentioned seed culture medium. Sterilization conditions are 120~125℃, sterilization for 15~25min...

Embodiment 2

[0080] The production strains were grown on the agar slope for 8 days and stored in a refrigerator at 4°C for 15 days. The pH was controlled to 6 during the fermentation process, and the dissolved oxygen level was controlled to 20-30% during the fermentation process. The fermentation process was 111 hours and consumed 72g / L glucose in total. The output of tamycin reached 4.0g / L. During the extraction, the fermentation broth was concentrated by the evaporation method, the methanol was removed by the vacuum evaporation method at 22°C, the pH was adjusted to 6.5 by adding acid to precipitate the dissolved natamycin, and the others were carried out as described in Example 1. The purity of the obtained natamycin precipitate reached 81%.

Embodiment 3

[0082] The fermenter is 200L, the liquid volume is 130L, and the seed culture is secondary culture. The secondary seeds were carried out in a 50L seed tank, with constant aeration of 0.5 v / v-min and constant stirring speed during the cultivation process, and the cultivation was carried out for 21 hours. Others are the same as in Example 1, fermentation for 168h, a total of 18kg glucose was consumed. The yield of natamycin was 5.4 g / L, and the purity of the obtained natamycin reached 85%.

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Abstract

The preparation method of natamycin belongs to a technique for producing and extracting natamycin by using luteofuscus spore streptomycete as strain and adopting fermentation process. It includes the following steps: adopting luteofuscus spore streptomycete, preparing spore suspension liquor, culturing seed, making femrentation, extracting, controlling pH value and dissolved oxygen level, and adopting glucose-feeding mode in batches in the period of fermentation to prepare natamycin. In order to raise its yield, adopting low-temp. evaporation methanol-removing process to extract natamycin.

Description

Technical field [0001] The invention belongs to a method for fermentative production of natamycin, and relates to a preparation technology for extracting natamycin by using a domestic culture medium with Streptomyces chrysosporium as a strain, and using controlled condition fermentation and evaporation to remove methanol. Background technique [0002] Natamycin (or pimaricin or tennessrin) is a polyene macrolide antifungal antibiotic with a molecular weight of about 666Dal, and the corresponding experimental molecular formula is C 33 H 47 NO 13 , Is an amphoteric substance with an isoelectric point of about 6.5. Natamycin has strong anti-biological activity against almost all fungi and is ineffective against bacteria. At present, Natamycin is widely used as a biological food preservative, and has related applications in medicine and other fields. Although people have realized that it has high-value antibiotic properties, its production cost is too high, which restricts domestic r...

Claims

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Application Information

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IPC IPC(8): A61P31/10C12P19/62
Inventor 杜连祥王敏路福平王建玲邬建国
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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