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Method used for preparing hydrogen via embedded bacteria fermentation

An embedding method and strain technology, applied in the field of biomass energy production, can solve the problem of affecting the mass transfer performance and light transmittance of embedded particles, affecting the substrate absorption efficiency, the hydrogen release rate, and the hydrogen production efficiency. It is impossible to achieve breakthroughs. It can improve the hydrogen production rate and substrate conversion rate, shorten the hydraulic retention time, good mass transfer performance and light transmittance.

Inactive Publication Date: 2016-06-29
COFCO GROUP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above inventions all use immobilized strains for simple anaerobic fermentation to produce hydrogen, while the theoretical yield of hydrogen production by simple dark fermentation is only 4mol / mol, so it is impossible to achieve a breakthrough in hydrogen production efficiency by only using one fermentation method of dark fermentation sexual enhancement
[0006] In addition, non-patent literature 1 uses polyvinyl alcohol with appropriate amount of sodium alginate, carrageenan, and activated carbon as embedding material, and boric acid and calcium chloride mixed solution as cross-linking agent for immobilization of photofermentative hydrogen-producing bacteria and dark However, the polyvinyl alcohol in the embedding material is a synthetic polymer material with poor mass transfer performance and has a certain negative impact on cell viability.
Carrageenan and activated carbon will also affect the absorption efficiency of the substrate and the release rate of hydrogen gas, thereby affecting the substrate utilization rate and hydrogen production rate
In addition, the diameter of the embedding particles prepared by this method is about 4mm, and larger diameters will also affect the mass transfer performance and light transmittance of the embedding particles, thereby affecting the hydrogen production efficiency

Method used

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  • Method used for preparing hydrogen via embedded bacteria fermentation
  • Method used for preparing hydrogen via embedded bacteria fermentation
  • Method used for preparing hydrogen via embedded bacteria fermentation

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preparation example Construction

[0056] For the preparation step (1) of the embedded strain of the present invention, those skilled in the art can appropriately select the type and concentration of the medium and cross-linking agent according to the description in this manual, and the hydrogen-producing strains produced by photofermentation and hydrogen production by dark fermentation strains. In one embodiment, the medium is an organic medium. Preferably, the organic medium is sodium alginate, polyacrylamide or a mixture of the two; the crosslinking agent is CaCl 2 . In one embodiment of the present invention, the dark fermentation hydrogen-producing strain is selected from the group consisting of Clostridium butyricum, Enterobacteraerogenes, Enterobacter cloacae, Clostridium thermocellum One or more of the group consisting of Clostridium barati and Bacillus thermophilus. In one embodiment of the present invention, the photofermentative hydrogen-producing bacteria are selected from Rhodospirillum, Rhodops...

Embodiment 1

[0083] Embodiment 1: Utilize the method for producing hydrogen by embedding strain fermentation

[0084](1) Preparation of embedded bacteria: Mix sodium alginate and deionized water at a mass volume ratio of 2:100 (g / ml, the same below) and heat to dissolve. When the solution is cooled to 30-40°C, follow the seaweed Sodium acid solution and bacteria solution are in a ratio of 1:2 (v / v) (the concentration of the bacteria is 2.0g / L), and the medium is evenly mixed with the dark fermentation hydrogen-producing bacteria solution and the light-fermented hydrogen-producing bacteria solution respectively. , and then injected with the aid of a syringe to a concentration of 1.5% CaCl 2 In the solution, immobilized dark-fermentation hydrogen-producing strains and immobilized light-fermentation hydrogen-producing strains are prepared. The dark-fermentation hydrogen-producing strain is Clostridium butyricum, and the light-fermentation hydrogen-producing strain is Rhodopseudomonas palustr...

Embodiment 2

[0090] Embodiment 2: Utilize the method for producing hydrogen by embedding strain fermentation

[0091] (1) Preparation of embedded bacteria: Mix polyacrylamide and deionized water at a mass volume ratio of 5:100 and heat to dissolve. When the solution is cooled to 30-40°C, the ratio of polyacrylamide to bacterial solution is 1: 3 (v / v) ratio (the strain concentration is 2.0g / L), the medium is uniformly mixed with the dark fermentation hydrogen-producing strain solution and the light-fermentation hydrogen-producing strain solution, and then injected with the aid of a syringe to a concentration of 3.0 % CaCl 2 In the solution, immobilized dark-fermentation hydrogen-producing strains and immobilized light-fermentation hydrogen-producing strains are prepared. The dark-fermentation hydrogen-producing strain is Enterobacter aerogenes, and the light-fermentation hydrogen-producing strain is Rhodobacter sphaericus.

[0092] (2) Heat gelatinization of starchy raw materials: put the...

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Abstract

The invention provides a method used for preparing hydrogen via fermentation of starchy raw materials by embedded bacteria. The method comprises following steps: 1, preparation of the embedded bacteria; 2, heating gelatinization of the starchy raw materials, 3, dark fermentation and hydrogen production, 4, photo fermentation and hydrogen production; and 5, purification. The method is high in hydrogen yield, hydrogen production rate, substrate utilization ratio, and energy conversion efficiency.

Description

technical field [0001] The invention belongs to the technical field of biomass energy production, and in particular relates to a method for fermenting starchy raw materials to produce hydrogen by using embedded bacteria. Background technique [0002] At present, global energy mainly relies on fossil fuels such as coal, oil and natural gas. The combustion of these fossil fuels has caused serious pollution to the global environment, and even threatens the health and survival of human beings themselves. Hydrogen energy is one of the ideal clean energy sources and has attracted widespread attention. As far as the current situation is concerned, most of the industrial production of hydrogen adopts physical and chemical methods, which consume a lot of electric energy and mineral raw materials, which makes the cost of hydrogen production too high, thus limiting the development of hydrogen energy. On the other hand, the method of using biomass organic matter as a substrate to produ...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N11/10C12N11/08C12N11/04C12R1/145C12R1/01
Inventor 苏会波程军周俊虎岑可法林海龙
Owner COFCO GROUP
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