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Dark-l fermentation integrated biological hydrogen production device

A technology of biological hydrogen production and photofermentation, which is applied in the direction of gas production bioreactors, microorganisms, and methods based on microorganisms, can solve the problems of low coupling hydrogen production efficiency, achieve unbalanced growth and metabolic rates, avoid shading effects, Effect of improving hydrogen production efficiency

Active Publication Date: 2013-06-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a dark-light fermentation integrated biological hydrogen production device in order to solve the problem of low hydrogen production efficiency of existing dark fermentation bacteria and light fermentation bacteria

Method used

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specific Embodiment approach 1

[0016] Specific implementation mode one: below in conjunction with attached figure 1 To further explain the specific embodiment: In this embodiment, the dark-light fermentation integrated biohydrogen production device includes a dark fermentation reaction zone 1, a filter membrane 3, a light fermentation reaction zone 4, a lighting system 6, a dark fermentation gas flow meter 7 and a light fermentation reaction zone. Gas flow meter 8, the biological hydrogen production reaction vessel is divided into a dark fermentation reaction zone 1 and a light fermentation reaction zone 4 through a filter membrane 3, a dark fermentation gas flow meter 7 is set on the dark fermentation reaction zone 1, and a dark fermentation gas flow meter 7 is set on the light fermentation reaction zone 4 There is a light fermentation gas flow meter 8, and an illumination system 6 is installed on one side of the light fermentation reaction zone 4, wherein the working volume of the dark fermentation reactio...

specific Embodiment approach 2

[0021] Specific embodiment 2: The dark fermentation bacteria added in the dark fermentation reaction zone 1 of this embodiment are Clostridium butyricum DSM10702, Enterobacter aerogenes DSM30053 or Ethanologenbacterium hit B49.

[0022] The bacteria described in this embodiment are all purchased.

specific Embodiment approach 3

[0023] Specific embodiment three: The photofermentation bacteria that can be added to the photofermentation reaction zone 4 of this embodiment are Rhodospirillum rubrum ATCC25903, Rhodopseudomonas RLD-119 or Rhodopseudomonas ( Rhodopseudomonas faecalis) RLD-53.

[0024] The bacteria described in this embodiment are all purchased.

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Abstract

The invention discloses a dark-photo fermentation integrated biological hydrogen production device, relates to a dark-photo fermentation integrated biological hydrogen production device and aims at the problem that the efficiency of hydrogen generation through coupling of the dark fermentation bacteria and the photo fermentation bacteria is low in the prior art. The biological hydrogen production device comprises a dark fermentation reaction region, a filter membrane, a photo fermentation region, an illumination system, a dark fermentation gas flow meter and a photo fermentation gas flow meter, wherein a biological hydrogen production reaction container is divided into the dark fermentation reaction region and the photo fermentation reaction region through the filter membrane; a dark fermentation gas flow meter is arranged on the dark fermentation reaction region; the photo fermentation gas flow meter is arranged on the photo fermentation reaction region; the illumination system is arranged at one side of the photo fermentation reaction region; and the working volume of the dark fermentation reaction region is smaller than that of the photo fermentation reaction region. The dark-photo fermentation integrated biological hydrogen production device disclosed by the invention has hydrogen yield of 3048.45ml H2 / L working volume and hydrogen generation rate of 4.08 mol H2 / mol glucose. The dark-photo fermentation integrated biological hydrogen production device is mainly applied to the biological hydrogen production field.

Description

technical field [0001] The invention relates to a dark-light fermentation coupled hydrogen production device. Background technique [0002] The diversity of microorganisms provides multiple pathways for biohydrogen production. Among them, dark fermentation bacteria can use complex macromolecular organic matter to produce H 2 , CO 2 and small molecule organic acids, etc. These small molecular organic acids provide abundant electron donors and carbon sources for photofermentation. Except for a very small number of light-fermenting bacteria that can use macromolecular organic matter for fermentation and will compete with dark-fermenting bacteria, the two microorganisms have almost no competition, but a relationship of cascade metabolism for substrates. Therefore, coupled hydrogen production is of great significance for improving substrate conversion efficiency and hydrogen production rate by combining microorganisms with different functions, coordinating the relationship be...

Claims

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Application Information

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IPC IPC(8): C12M1/107C12R1/145C12R1/01
CPCC12M21/04C12M23/34C12M29/04C12M31/02
Inventor 任南琪谢国俊刘冰峰丁杰任宏宇
Owner HARBIN INST OF TECH
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