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Method for fermenting starchy raw material with mixed strain to produce hydrogen

A technology of starchy raw materials and mixed strains, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve difficult hydrogen production process amplification, industrial application, nutrient conditions, growth environment and process parameter regulation requirements Very high, affecting the effect of hydrogen production, etc.

Inactive Publication Date: 2016-06-29
COFCO GROUP +1
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  • Abstract
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Problems solved by technology

[0005] In order to improve the hydrogen production rate and energy utilization rate, non-patent literature 1 uses cassava starch as the substrate, uses cow manure composting mixed strains as dark fermentation mixed strains, and uses Rhodobacter sphaeroides as photofermentation strains for dark fermentation. - Light-coupled fermentation for hydrogen production. However, this study uses a single strain of bacteria during photosynthetic fermentation, which requires high regulation of nutritional conditions, growth environment and process parameters. Minor changes in the above process parameters will affect the hydrogen production effect
In addition, the working volume of this research is only 30-40mL, which is too far away from small-scale, pilot-scale research and actual production, and it is difficult to directly scale up or industrialize the hydrogen production process.

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  • Method for fermenting starchy raw material with mixed strain to produce hydrogen

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Effect test

Embodiment 1

[0127] Among them, Δt is the increment (h) at time t, and when Δt approaches 0, it is the hydrogen production rate at time t. When calculating the maximum hydrogen production rate, the hydrogen production curve is made according to the total hydrogen production at different times, and then the curve is derived to obtain the hydrogen production rate at different times, and the maximum hydrogen production rate can be obtained accordingly. Embodiment 1: Utilize mixed strain, carry out two-step method fermentation to produce hydrogen to cassava starchy raw material

[0128] The cassava starch solution with a concentration of 10 g / L was placed in a high-pressure steam cooker at 115° C., and heated and gelatinized for 15 minutes. Then proceed as follows:

[0129] (1) Liquefaction: Add α-amylase to the cassava starch solution after heating and gelatinizing in step (1) for liquefaction; the mass ratio of the added α-amylase to tapioca starch is 0.05, and the control liquefaction temp...

Embodiment 2

[0135] Example 2: Using mixed strains to carry out two-step fermentation of corn starch raw materials to produce hydrogen

[0136] The cornstarch solution with a concentration of 20g / L was placed in a high-temperature oven at 120°C, and heated for gelatinization for 10 minutes. Then proceed as follows:

[0137] (1) Liquefaction: Add α-amylase to the corn starch solution after heating and gelatinizing in step (1) for liquefaction; the mass ratio of the added α-amylase to corn starch is 0.03, and the liquefaction temperature is controlled to be 58°C , the liquefaction pH is 6.2, and the time is 4 hours.

[0138] (2) Saccharification: Add glucoamylase to the solution liquefied in step (1) for saccharification, after saccharification, inactivate the glucoamylase by heating at 100°C for 10 minutes; the mass ratio of the added glucoamylase to starch is 0.03, the saccharification temperature is controlled at 40°C, the saccharification pH is 4.3, and the time is 16 hours.

[0139] ...

Embodiment 3

[0143] Example 3: Using mixed strains to carry out two-step fermentation of wheat starchy raw materials to produce hydrogen

[0144] Place the wheat starch solution with a concentration of 30 g / L in a high-pressure steam cooker at 105° C., and heat and gelatinize for 25 minutes. Then proceed as follows:

[0145] (1) Liquefaction: Add α-amylase to the wheat starch solution after heating and gelatinizing in step (1) for liquefaction; the mass ratio of the added α-amylase to wheat starch is 0.03, and the controlled liquefaction temperature is 62°C , the liquefaction pH is 5.8, and the time is 1.5 hours.

[0146] (2) Saccharification: Add glucoamylase to the solution liquefied in step (1) for saccharification, after saccharification, inactivate the glucoamylase by heating at 100°C for 10 minutes; the mass ratio of the added glucoamylase to starch is 0.03, the saccharification temperature is controlled at 58°C, the saccharification pH is 4.5, and the time is 20 hours.

[0147] (...

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Abstract

The invention provides a method for two-step fermentation of a starchy raw material with a mixed strain to produce hydrogen. The method comprises the steps of: (1) liquefaction; (2) saccharification; (3) dark fermentation to produce hydrogen; (4) photo-fermentative hydrogen production; and (5) purification. The method provided by the invention has excellent hydrogen production rate, hydrogen production velocity, substrate utilization rate and energy conversion efficiency.

Description

technical field [0001] The invention belongs to the technical field of biomass energy production, and in particular relates to a method for producing hydrogen by using mixed strains to ferment starchy raw materials in two steps. Background technique [0002] The use of fossil fuels will cause global warming, which will have a huge impact on the environment, and hydrogen is generally considered to be an ideal alternative energy source for fossil fuels due to its clean, high-energy and recyclable characteristics. At present, the production of hydrogen by fermentation of biomass feedstock is attracting more and more attention. This is because biomass fermentation can not only provide clean hydrogen energy, but also eliminate environmental pollution caused by biomass organic waste. Its mild reaction conditions and low energy requirements have irreplaceable advantages. [0003] In the field of biomass fermentation hydrogen production, expensive raw material costs and low hydrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12R1/145C12R1/01
Inventor 苏会波程军周俊虎岑可法林海龙
Owner COFCO GROUP
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