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Method utilizing hydrogen-producing acetogens and electricigens to enhance biological hydrogen production efficiency

A technology of hydrogen-producing acetogenic bacteria and biological hydrogen production, applied in the direction of fermentation, etc., can solve the problems of development limitation of fermentation hydrogen production, unstable operation of dark fermentation reactor, incomplete conversion of substrate, etc., and achieve low price and high hydrogen production Efficiency improvement and cost saving effects

Inactive Publication Date: 2014-06-18
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology

In existing studies, it was found that during the dark fermentation reaction, the volatile fatty acids produced could not be further spontaneously converted into H by hydrogen-producing acetogenic bacteria. 2 , which can easily lead to unstable operation of dark fermentation reactors and incomplete conversion of substrates, which limits the development of fermentative hydrogen production

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  • Method utilizing hydrogen-producing acetogens and electricigens to enhance biological hydrogen production efficiency

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Embodiment 1

[0014] The enrichment reactor for hydrogen-producing acetogenic bacteria is carried out by inoculating the aerobic activated sludge of the secondary sedimentation tank with the ABR reactor; the enrichment of electrogenic bacteria is carried out through the MFC reactor, and then introduced into the microbial electrolysis cell (MEC), Finally, the effluent of the ABR reactor was used as the substrate of MEC to realize the combined hydrogen production of hydrogen-producing acetogenic bacteria and electrogenic bacteria.

[0015] 1. Enrichment of hydrogen-producing acetogenic bacteria and electrogenic bacteria

[0016] Preparation of ABR feed water: add a certain amount of carbohydrates to tap water, and add 5.73g NH at the same time 4 Cl, 1.32g KH 2 PO 4 , 30g NaHCO 3 , while adding 1ml / L trace element solution to prepare organic wastewater; the final control concentration of chemical oxygen demand (COD) of organic wastewater is 4600mg / L, pH=7.10.

[0017] Preparation of MEC st...

Embodiment 2

[0030]Coupling hydrogen-producing acetogenic bacteria and electrogenic bacteria to achieve cascade organic matter degradation and deep hydrogen production. Among them, hydrogen-producing acetogenic bacteria and electrogenic bacteria were respectively inoculated into the same anaerobic reactor ABR with pure strains. The aerobic activated sludge of the secondary sedimentation tank was inoculated into the first chamber of the ABR reactor to degrade the organic matter initially, and the pure hydrogen-producing acetogenic bacteria were inoculated in the second chamber by using the plug-flow characteristics of the ABR. The products generated in the first compartment are degraded into H 2 ; Finally, inoculate the pure-bred electrogenic bacteria in the third compartment, and use the cathode and anode connected in the compartment, under the catalysis of the additional voltage, further degrade and convert the terminal substrate volatile fatty acid produced in the second compartment into...

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Abstract

The invention discloses a method utilizing hydrogen-producing acetogens and electricigens to enhance the biological hydrogen production efficiency. The method comprises the following steps: utilizing hydrogen-producing acetogens and electricigens to deeply degrade the organisms, modulating the operation parameters of the dark fermentation technology so as to high efficiently accumulate acetic acid in the end products of the water-generating hydrogen-producing acetogens, taking the generated water as the substrate of an electric catalysis reactor, modulating the additional voltage value, taking the Ni nano particles as the catalyst, the stainless steel as the negative pole, and carbon cloth as the positive pole, and utilizing the extracellular electron transfer effect of the electricigens to further degrade the end products of the hydrogen-producing acetogens to convert the end products into hydrogen gas; thus the phenomenon of fermentation obstacle is weakened, high efficient hydrogen production by utilizing hydrogen-producing acetogens and electricigens is achieved, hydrogen production and pollution treatment are integrated, and the method has the characteristics of cost saving, little energy consumption, and high hydrogen production efficiency.

Description

technical field [0001] The patent of the present invention relates to a method for enhancing the efficiency of biological hydrogen production by combining hydrogen-producing acetogenic bacteria and electrogenic bacteria. It belongs to the technical field of biological hydrogen production methods, and is a specially designed biological hydrogen production method that reduces the phenomenon of "fermentation barrier" and improves hydrogen production rate by combining hydrogen-producing acetogenic bacteria and electrogenic bacteria. Background technique [0002] Hydrogen production through anaerobic fermentation technology, including light fermentation and dark fermentation technology, utilizes the metabolism of microorganisms, integrates pollution control and production capacity, has the advantages of cleanliness, energy saving and realization of waste resources, and has been widely valued and developed. . Among them, the photofermentation technology uses algae and photosynthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
Inventor 朱葛夫吴婷婷刘超翔
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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