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Method for producing hydrogen by dark-light zymolysis couple

A fermented liquid and hydrogen technology, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of unutilized organic acids, low conversion efficiency of substrates, high cost of hydrogen production, etc., and achieve substrate conversion High efficiency, realization of circular economy, and low cost of hydrogen production

Inactive Publication Date: 2008-01-23
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of low substrate conversion efficiency, high hydrogen production cost, unusable organic acids produced by dark fermentation, and environmental pollution in the existing dark fermentation hydrogen production methods, new processes and technologies need to be provided in this field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Biological hydrogen production from canteen kitchen waste

[0050] 1. Pretreatment of hydrogen-producing bacteria: Take natural composted cow dung and boil it at 100°C for 15 minutes.

[0051] 2. Configuration of the dark fermentation liquid of kitchen waste in the canteen: take back the kitchen waste (mainly rice, vegetables, fruit peels, and various meat and bones discarded by the canteen) directly from the student canteen, and measure its water content It is about 65%-70%, and then directly use a mixer to mix the kitchen waste with water and mix it evenly. Then add some nutrients to the mixed kitchen waste: KH 2 PO 4 1.5g / L, Na 2 HPO 4 12H 2 O 4.2g / L, glutamic acid 1g / L, yeast extract 2g / L, MgCl 2 0.18g / L, FeSO 4 0.1g / L, adjust its pH to 6.0. Measure the concentration of the prepared kitchen waste domestic waste fermentation liquid, 30g / L-TS, 54.2g / L-COD, 25.2g / L-TOC, total nitrogen 1.04g / L, total phosphorus 200mg / L, NH4 + 98.4mg / L, COD:N:P is ...

Embodiment 2

[0057] Example 2 Enlargement of reaction volume for hydrogen production from kitchen waste

[0058] The composition and concentration of the pretreated cow dung mixed live flora and domestic waste dark fermentation are exactly the same as in Example 1, except that the fermentation reactor is enlarged and carried out in a 5L glass reactor, and the volume of the fermentation liquid is 3.5L , the stirring speed is 450rpm / min, the temperature is controlled in the range of 36-39°C, and hydrogen is produced by dark fermentation under normal pressure.

[0059] Hydrogen production begins 7-8 hours after inoculation. 15 minutes after the start of hydrogen production, the hydrogen content accounted for more than 50% of the mixed gas (H2 and CO2), and the highest accounted for 65% of the mixed gas. Until the end of hydrogen production, the hydrogen content did not fall below 50%. The maximum hydrogen production rate is 679ml / L h, and the hydraulic duration of the whole hydrogen producti...

Embodiment 3

[0062] Example 3 Biological hydrogen production from kitchen waste with different initial concentrations

[0063] The composition of the pretreated cow dung mixed live flora and domestic waste dark fermentation is exactly the same as that of Example 1 and Example 2, except that the initial concentration of the kitchen waste dark fermentation liquid is changed to 18g / l-TS, 35.6g / l-COD, 20g / l-TOC, total nitrogen 0.92g / L, total phosphorus 0.45g / L, NH4 + 98.1mg / L, and the initial pH value was adjusted to 6.0, carried out in a 250ml glass reactor (fermentation broth volume 180ml), and the rest of the dark fermentation hydrogen production conditions were the same as in Examples 1 and 2. The hydrogen production duration of each batch of dark fermentation is about 3 days, and the highest hydrogen production rate reaches 640mlH 2 / L h, the hydrogen content is up to 68% of the mixed gas content (hydrogen and carbon dioxide), and every gram of dry weight kitchen waste produces 182ml hy...

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Abstract

The invention provides a method for producing hydrogen by fermentation, in particular to a waste biomass clean and turning into hydrogen energy technology. The invention comprises the following steps: firstly taking the carbon-contained organic waste as the fermentation substrate, the pretreated mixture bacteria of natural livestock manure is dark fermented to get the fermentation liquid of hydrogen and organic acid. Then the photosynthetic bacteria are applied to anaerobicly ferment the fermentation liquid gotten from the above step to produce hydrogen. The invention has advantages of quick hydrogen production, high substrate conversion efficiency, low hydrogen producing cost and standard fermentation liquid emission.

Description

technical field [0001] The invention belongs to the technical field of bioenergy. It specifically involves the use of natural microbial communities as the source of hydrogen-producing bacteria, using carbon-containing organic waste, especially waste biomass such as kitchen waste and ethanol fermentation wastewater, as raw materials, and a clean production method for producing hydrogen through coupling of dark fermentation and light fermentation . Background technique [0002] At present, the reserves of fossil fuels (oil, natural gas and coal), which are the arteries of the global economy, are very limited, and the use of fossil energy is the main cause of environmental pollution and greenhouse effect. It is imperative to develop renewable and clean energy. [0003] Compared with traditional energy sources, hydrogen has the advantages of high energy density (three times that of gasoline), high thermal conductivity, high heat conversion efficiency, and low transportation co...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12P39/00C12R1/01A62D3/00
Inventor 周志华陶用珍吴永强
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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