The application belongs to the technical field of
biotechnology, and particularly relates to a gel
microsphere loaded with probiotics and a preparation method and application thereof. The gel
microsphere comprises core material and composite
wall material, the core material is probiotics, the composite
wall material is composed of
sodium alginate, beta-conglycinin and
thio-oxidized
konjac glucomannan, and a 3D printing microfluidic technology is combined to construct an embedding
system, so that precise preparation of the
microsphere is realized, the particle size of the microsphere can be precisely controlled by adjusting parameters such as flow rate and
viscosity, and the embedding microsphere with high monodispersity and uniform morphology is obtained. The synergistic effect of the composite
wall material in the application not only enhances the stability of the embedding structure, but also endows the microsphere with pH /
enzyme dual response characteristics and intestinal mucosal adhesion ability, and solves the problems of poor embedding protection effect and insufficient targeting of single wall material.