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Sodium hyaluronate irrigation solution for nonbacterial cystitis treatment

A non-bacterial, lavage solution technology, applied in the field of biomedicine, can solve the problems of easy recurrence of the disease, single drug therapy and other problems

Inactive Publication Date: 2015-11-11
SUZHOU JIOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, since this therapy is more effective than a single drug, although it can effectively improve the discomfort symptoms of non-bacterial bladder patients in the short term, the disease is prone to relapse, and the long-term efficacy needs to be accumulated in clinical research experience

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation and Characterization of Hyaluronic Acid-Cysteamine (HA-Cym) and or Hyaluronic Acid-Cysteine ​​(HA-Cys)

[0028] Weigh 0.4g of HA (molecular weights are 750,000, 1 million, and 1.5 million Daltons), add 100mL water to swell overnight to form a viscous solution, add EDAC and NHS with a final concentration of 50mmol / L, and add 1mol / L L of HCl solution to adjust the pH to 5.5, stir and react for 45 minutes at room temperature in the dark, weigh 0.8g cysteamine (Cym) or cysteine ​​(Cys), respectively add to the reaction system, add 1mol / L NaoH solution Adjust the pH to 5.0, stir and react overnight at room temperature in the dark, then dialyze with pH 5.0 HCl solution, pH 5.0 HCl solution containing 1% (mass percent) NaCl, and pH 5.0 HCl solution at 4°C in the dark for 2 HA-Cym and HA-Cys polymers were prepared by freeze-drying. The structure of the obtained polymer was confirmed by H NMR, infrared and DSC. Molecular weight was determined by multi-ang...

Embodiment 2

[0029] Embodiment 2 sodium hyaluronate molecular weight determination

[0030] The molecular weight and molecular weight distribution of polymer compounds are important factors affecting their physical properties and biological functions. Multi-angle laser light scattering instrument combined with size exclusion chromatography (MALLS-GPC) technology combines the characteristics of GPC method and light scattering method, and can quickly and accurately determine the absolute weight-average molecular weight, number-average and Z of polymers. Average molecular weight and molecular weight distribution, the measurement does not depend on pump speed, polymer standards or molecular conformation, the results are accurate, reliable and reproducible. Hyaluronic acid and its salts, thiolated hyaluronic acid and its salts, chondroitin sulfate, dermatan sulfate and heparin can all be used to determine molecular weight and molecular weight distribution range by this method.

[0031] Chromat...

Embodiment 3

[0033] The preparation of embodiment 3 lavage fluid

[0034] prescription:

[0035] Sodium Hyaluronate 0.1%

[0036] Hyaluronic Acid-Cysteamine 0.01%

[0037] Chondroitin Sulfate 0.5%

[0038] Dermatan Sulfate 0.5%

[0039] Heparin 0.1%

[0040] Glucosamine 2%

[0041] Sodium hydroxide to adjust pH to 6.0

[0042] Methylparaben and Phenoxyethanol (1:1) 0.1%

[0043] water balance

[0044] Preparation:

[0045] 1. The prescribed amount of sodium hyaluronate (molecular weight 750,000 daltons), hyaluronic acid-cysteamine (molecular weight 750,000 daltons), chondroitin sulfate (molecular weight 22,000 daltons), dermatan sulfate ( Molecular weight 22,000 Daltons), heparin (molecular weight 15,000 Daltons) and heparin are dispersed in water and stirred to dissolve;

[0046] 2. Mix the above two solutions evenly under stirring, add the prescribed amount of sodium hydroxide to adjust the pH of the temperature-sensitive gel to 6.0;

[0047] 3. Add 0.1% activated carbon, stir...

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PUM

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Abstract

The invention belongs to the field of biological medicines, and relates to a bladder irrigation solution which is used for treating the effective concentration of nonbacterial cystitis, is in contact with the internal surface epithelium and relevant structures of the bladder of a patient suffering from the nonbacterial cystitis to prevent and treat the nonbacterial cystitis and contains sodium hyaluronate. The bladder irrigation solution comprises a water-soluble mucous membrane adhesive material, an active component and a medically acceptable carrier and / or an additive. The water-soluble mucous membrane adhesive material is prepared from hyaluronic acid-cysteine and salt thereof, and the molecular weight is not less than 50,000 Dalton, and the use amount is smaller than 1 percent; the active component comprises glycosaminoglycan and / or amino monosaccharide; glycosaminoglycan is one or a combination of several of salt, chondroitin sulfate and dermatan sulfate of any proportion; amino monosaccharide is a combination of one or two of glucosamine and acetylglucosamine of any proportion. The irrigation solution contains the medically acceptable carrier and / or an additive which comprises an osmotic pressure adjusting agent, a pH adjusting agent, a preservative and water.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a lavage solution for preventing and treating non-bacterial cystitis and a preparation method thereof. Background technique [0002] Nonbacterial cystitis is a kind of chronic bladder inflammation with complicated etiology, difficult diagnosis, and difficult to eradicate. The main clinical manifestations are frequent urination, urgency, nocturia, suprapubic bladder pain, and some patients may be accompanied by painful urination and hematuria. Urine cultures were negative. Common classifications include interstitial cystitis, radiation cystitis, chemical cystitis, and other chronic bladder inflammations of nonbacterial causes. [0003] Interstitial cystitis (interstitialcystitis, IC) is a syndrome of unknown etiology. In the early stage of pathological changes, the appearance under cystoscopy is normal or only partially congested. , There may be a variety of inflammatory cell infiltra...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K47/36A61K31/737A61K31/7008A61P13/10
Inventor 张自强
Owner SUZHOU JIOU BIOTECH CO LTD
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