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Preparation method of short amylase-insulin or short amylase-insulin-procyanidine nanocomposite

A short amylose, nanocomposite technology, applied in nanomedicine, nanotechnology, nanotechnology and other directions, can solve problems such as increasing the physical and psychological burden of patients, achieve improved bioavailability, prolong residence time, overcome mucus layer The effect of the barrier

Active Publication Date: 2017-09-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Subcutaneous injection of insulin preparations is the main method currently used in the treatment of diabetes. It has a faster hypoglycemic effect and higher drug availability. However, due to the rapid release of the drug, frequent injections are required, which increases the physical and psychological burden of the patient.

Method used

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  • Preparation method of short amylase-insulin or short amylase-insulin-procyanidine nanocomposite

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of short amylose-insulin nanocomposite, described nanocomposite is made through the following steps:

[0027] (1) Preparation of short amylose: prepare waxy cornstarch milk with a mass percentage of 15%, and make it completely gelatinized in a water bath at 100°C for 30 minutes, then cool down to 58°C, and add 100U / mL pullulanase to it, Enzymolysis for 6 hours, centrifugation, enzyme inactivation, and freeze-drying to obtain short amylose;

[0028] (2) Preparation of short amylose solution: weigh 0.1 g of short amylose prepared in step (1) and add it to 10 mL of deionized water, sonicate for 5 minutes, then 100 ° C water bath for 30 minutes to make it completely gelatinized, and cool down to room temperature. A short amylose solution with a mass concentration of 1% was prepared;

[0029] (3) Preparation of insulin solution: Weigh 0.01g of insulin powder and dissolve it in 10mL of hydrochloric acid with pH=2 to prepare a 1‰ insulin solution;

[0030] (4) Prepara...

Embodiment 2

[0032] A method for preparing a short amylose-insulin-proanthocyanidin nanocomposite, characterized in that the preparation method comprises the following steps:

[0033] (1) Preparation of short amylose: prepare waxy cornstarch milk with a mass percentage of 15%, and make it completely gelatinized in a water bath at 100°C for 30 minutes, then cool down to 58°C, and add 100U / mL pullulanase to it, Enzymolysis for 6 hours, centrifugation, enzyme inactivation, and freeze-drying to obtain short amylose;

[0034] (2) Preparation of short amylose solution: weigh 0.1 g of short amylose prepared in step (1) and add it to 10 mL of deionized water, sonicate for 5 minutes, then 100 ° C water bath for 30 minutes to make it completely gelatinized, and cool down to room temperature. A short amylose solution with a mass concentration of 1% was prepared;

[0035] (3) Preparation of insulin solution: Weigh 0.01g of insulin powder and dissolve it in 10mL of hydrochloric acid with pH=2 to prepa...

Embodiment 3

[0039]A kind of short amylose-insulin nanocomposite, described nanocomposite is made through the following steps:

[0040] (1) Preparation of short amylose: prepare waxy cornstarch milk with a mass percentage of 15%, and make it completely gelatinized in a water bath at 100°C for 30 minutes, then cool down to 58°C, and add 100U / mL pullulanase to it, Enzymolysis for 6 hours, centrifugation, enzyme inactivation, and freeze-drying to obtain short amylose;

[0041] (2) Preparation of short amylose solution: Weigh 0.1 g of short amylose prepared in step (1) and add it to 10 mL of deionized water, sonicate for 5 minutes, then make it completely gelatinized in a water bath at 100° C. for 30 minutes, and cool down to room temperature. A short amylose solution with a mass concentration of 1% was prepared;

[0042] (3) Preparation of insulin solution: Weigh 0.01g of insulin powder and dissolve it in 10mL of hydrochloric acid with pH=2 to prepare a 1‰ insulin solution;

[0043] (4) Pre...

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Abstract

The invention discloses a preparation method of short amylase-insulin or short amylase-insulin-procyanidine nanocomposite. The preparation method comprises the steps of mixing short amylase with insulin or mixing short amylase, insulin and procyanidine, and carrying out retrogradation, so as to obtain short amylase-insulin or short amylase-insulin-procyanidine nanocomposite. The short amylase-insulin or short amylase-insulin-procyanidine nanocomposite is relatively small in particle size and can tightly adhere to a membrane adhesion layer of a small intestine, so that the standing time in the stomach and intestine is prolonged, meanwhile, a mucous layer barrier, an enzyme barrier and an epithelial barrier existing in an alimentary system can be overcome, and the bioavailability of oral insulin is increased.

Description

technical field [0001] The invention relates to the technical field of nanocomposite materials, in particular to a method for preparing nanocomposites by mixing short-chain starch with insulin or short-chain starch with insulin and proanthocyanidins. Background technique [0002] Subcutaneous injection of insulin preparations is the main method currently used in the treatment of diabetes. It has a faster hypoglycemic effect and higher drug availability. However, due to the rapid release of the drug, frequent injections are required, which increases the physical and psychological burden of the patient. . There is also a big difference between the way of injection administration and the secretion of insulin in the body under normal physiological conditions; insulin enters the human circulation directly after injection; under normal physiological conditions, insulin secreted by pancreatic islets first enters the hepatic portal vein system , most of which are taken up by the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61K47/61A61K47/54A61P3/10B82Y5/00
CPCA61K9/0002A61K38/28B82Y5/00
Inventor 洪雁姬娜顾正彪程力李兆丰李才明
Owner JIANGNAN UNIV
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