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Microbial test strip and application thereof

A technology of microorganisms and test strips, applied in the biological field, can solve the problems of low detection results, easy diffusion of sample liquid to the outside of the culture area, complicated production process, etc., and achieve the effects of accurate results, high accuracy and simple production process

Inactive Publication Date: 2016-09-21
孙百莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3M test strips are such products, but there are disadvantages such as cumbersome manufacturing process, and the sample liquid is easy to diffuse to the outside of the culture area after adding the sample, which makes the test result low.

Method used

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  • Microbial test strip and application thereof
  • Microbial test strip and application thereof
  • Microbial test strip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, microbial test sheet

[0044] Structural representation of the microbial test piece of the present invention Figure as shown 1 and picture 2 shown.

[0045] The microbial test piece provided by the present invention comprises a coating 1, an adhesive tape 3 adhered with a color developing culture system and a hydrophobic sheet 2 between the two; the hydrophobic sheet is provided with a through hole, and one surface of the hydrophobic sheet It is covered on the chromogenic culture system, and the through hole corresponds to the position of the chromogenic culture system; the film covers the other surface of the hydrophobic sheet. The surface of the hydrophobic sheet with glue is adhesively connected with the surface of the adhesive tape attached with the chromogenic culture system, and the chromogenic medium on the adhesive tape is exposed from the hole; one side of the film is connected to one side of the other surface of the hydrophobic sheet 2 Adh...

Embodiment 2

[0050] Embodiment 2, the preparation and application of the total number of microbial colonies test piece

[0051] 1. The production of the total number of microbial colony test pieces

[0052] 1. Preparation of adhesive tape adhered with chromogenic culture system

[0053] Mix 2.00g sodium alginate (Henan Dewang Chemical Industry Co., Ltd.; product number: 9005-38-3) and 1.05g plate counting chromogenic medium to obtain a chromogenic culture system, and then adhere the chromogenic culture system to the On the tape, the tape with the chromogenic culture system adhered was obtained.

[0054]The above plate counting chromogenic medium is obtained by mixing 1.00g bacterial culture medium and 0.05g indicator TTC (product number T8877 of Sigma Company); the bacterial culture medium is composed of 5.00g tryptone, 2.50g yeast extract powder, and 1.00g glucose. Mix evenly after weighing, and then weigh 1.00g from the mixture.

[0055] 2. Preparation of hydrophobic sheet with throug...

Embodiment 3

[0072] Embodiment 3, the making and application of coliform test piece

[0073] 1. Production of coliform test strips

[0074] 1. Preparation of adhesive tape adhered with chromogenic culture system

[0075] Mix 2.00g of sodium alginate (Henan Dewang Chemical Industry Co., Ltd.; product number: 9005-38-3) and 1.05g of microbial chromogenic medium to obtain a chromogenic culture system, and attach the chromogenic culture system to the adhesive tape , to obtain a tape adhered with the chromogenic culture system.

[0076] The above microbial chromogenic medium was obtained by mixing 1.00 g of VRB medium and 0.05 g of indicator TTC (product number T8877 of Sigma Company). VRB medium is composed of 7.00g peptone, 3.00g yeast extract powder, 5.00g sodium chloride, 10.00g lactose, 1.50g bile salt, 0.03g neutral red, weighed accurately and mixed evenly, and then weighed accurately from the mixture 1.00g.

[0077] 2. Preparation of hydrophobic sheet with through holes

[0078] The...

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Abstract

The invention discloses a microbial test strip and application thereof. The microbial test strip comprises a covering membrane, an adhesive part to which a chromogenic culture system is adhered and an intermediate membrane positioned between the covering membrane and the adhesive part, wherein the adhesive part to which the chromogenic culture system is adhered comprises an adhesive part and the chromogenic culture system adhered thereto; the chromogenic culture system consists of cold-water-soluble gel and a microbial chromogenic culture medium; a through hole is formed in the intermediate membrane; the chromogenic culture system is covered by one surface of the intermediate membrane, and the through hole corresponds to the position of the chromogenic culture system; the other surface of the intermediate membrane is covered by the covering membrane. According to the microbial test strip, work of vessel cleaning, culture medium preparation, waste treatment and the like of an operator is eliminated, and the microbial test strip is convenient to operate, available when needed and high in product repeatability and accuracy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of rapid detection of microorganisms, in particular to a microorganism test piece and its application. Background technique [0002] At present, the national standard method is mainly used for microbial detection in my country, and traditional detection techniques are used, such as plate counting method, MPN method, and biochemical identification. Disposal of waste, etc., not only has high requirements for instruments and equipment, but also has high requirements for the skills and experience of operators. Due to the many detection links and easy pollution, the accuracy of the detection results is not easy to control. [0003] The test piece has won the favor of the majority of testing personnel for its advantages of simple production process, easy operation, accurate results, and short detection time. The test piece does not need to be prepared before the experiment, whic...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/06
CPCC12Q1/06
Inventor 孙百莉迟俊
Owner 孙百莉
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