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Aerobic denitrification methylobacterium phyllosphaerae and application thereof

A technology of Methylobacterium nitrate and aerobic denitrification, which is applied in the field of environmental microorganisms, can solve the problems of large area occupied by sewage treatment structures, unsatisfactory total nitrogen removal effect, high investment and operating costs, and overcome the problems of treatment efficiency and economy. Applicable, shortened start-up time, stable biological activity

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional biological denitrification includes two processes of aerobic nitrification and anaerobic denitrification, which require the separation of nitrification and denitrification, which has many disadvantages such as lengthy process, large footprint of sewage treatment structures, high investment and operating costs, etc.
In response to the above problems, some researchers tried to add nitrifying bacteria and denitrifying bacteria into the same reactor. Although the problems in the traditional process were partially solved, the total nitrogen removal effect was often unsatisfactory due to the difference between the two bacteria.

Method used

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  • Aerobic denitrification methylobacterium phyllosphaerae and application thereof
  • Aerobic denitrification methylobacterium phyllosphaerae and application thereof
  • Aerobic denitrification methylobacterium phyllosphaerae and application thereof

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Effect test

preparation example Construction

[0029] ③Preparation of seed solution: Inoculate the purified and cultured strain SDN-3 into 150 mL of re-screened liquid medium, and culture at 30°C to 35°C for 3 days. The amount of bacteria in the subsequent wastewater treatment was added according to the amount of 10g / L after centrifugation of the seed culture solution.

[0030] ④ Investigation of carbon source utilization ability: Sodium succinate, asparagine, sodium citrate, sodium acetate, ethanol and methanol, which are more commonly used in screening aerobic denitrifying bacteria, were selected as carbon sources for investigation. The results showed that the strain SDN-3 could use a variety of carbon sources, especially methanol as the carbon source for aerobic denitrification, the removal rate of total nitrogen was 90%, and the removal rate of COD was 97%. The total nitrogen removal effect of SDN-3 treatment of wastewater containing different carbon sources is shown in the appendix figure 1 .

[0031] ⑤Heterotrophi...

Embodiment 1

[0033] Examination of the aerobic denitrification performance of Methylobacterium SDN-3.

[0034] The simulated wastewater I was prepared, with nitrate nitrogen as the only nitrogen source, the initial concentration was 277mg / L, and the mass ratio of carbon to nitrogen was 5.3. Dissolved oxygen (DO) was 0.5 mg / L, temperature was 24°C, and pH was 7.0 for 24 hours. The removal rate of nitrate nitrogen is greater than 95%; the removal rate of total nitrogen is greater than 90%, and the average removal rate of total nitrogen is 10.4mg / (L·h); the removal rate of COD is greater than 85%.

[0035] Simulated Wastewater I: Methanol 5.0mL, KNO 3 2.0g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5.

Embodiment 2

[0037] Heterotrophic nitrification-aerobic denitrification performance of Methylobacterium SDN-3.

[0038] Prepare simulated wastewater II, with ammonia nitrogen as the only nitrogen source, with an initial concentration of 265 mg / L and a carbon-to-nitrogen mass ratio of 3.4. Treat for 26 hours under the conditions of DO 2.5mg / L, temperature 35°C, and pH 8.5. The removal rate of ammonia nitrogen and total nitrogen is greater than 90%, the average removal rate of total nitrogen is 9.2mg / (L·h); the removal rate of COD is greater than 99%.

[0039] Simulated Wastewater II: Methanol 3.0mL, Ammonium Sulfate 1.25g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5.

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Abstract

The invention relates to an aerobic denitrification methylobacterium phyllosphaerae strain and application thereof. The aerobic denitrification strain is methylobacterium phyllosphaerae SDN-3CGMCC No.3660. The strain can be used for carrying out aerobic denitrification by using nitrate nitrogen and can also be used for carrying out hetetrophic nitrification-aerobic denitrification by using ammonia nitrogen, under aerobic and limited-oxygen conditions. The strain is stable in genetic character, and can be used for realizing synchronous nitrification-denitrification and independently completingthe whole process of biological denitrification. The strain is used for treating various nitrogen-containing wastewater by needing only one aerobic stage, the process is simple and the denitrification efficiency is high.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a Methylobacterium phyllosphaerae used for biological denitrification and its application. The methylobacterium of the invention has strong denitrification performance under both aerobic and oxygen-limited conditions, and is especially suitable for denitrification in ammonia-containing wastewater during simultaneous nitrification and denitrification. Background technique [0002] With the increasing nitrogen pollution in water bodies, denitrification has become the focus and hotspot of water treatment research. At present, it is generally believed that biological denitrification is one of the more economical and effective methods to remove nitrogen pollution from wastewater. Traditional biological denitrification includes two processes of aerobic nitrification and anaerobic denitrification, which require the separation of nitrification and denitrification, which has m...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02
CPCY02W10/10
Inventor 李志瑞高会杰黎元生许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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