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Method for fermentation-membrane separation combined production of long-chain dicarboxylic acid

A long-chain dibasic acid and membrane separation technology, applied in the field of fermentation engineering, can solve problems such as low production efficiency, and achieve the effects of recycling, reducing difficulty, reducing material consumption and cultivation time

Active Publication Date: 2015-08-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of low production efficiency in the process of microbial synthesis of long-chain dibasic acids, the technical problem solved by the present invention is: during the fermentation process, membrane technology is used to reflow the bacteria and separate the synthesized high-concentration dibasic acids to reduce the production of dibasic acids in the product. The inhibitory effect of acid on its synthesis process; increase the cell density of the fermentation process, shorten the fermentation cycle, and increase the production intensity of the synthetic dibasic acid; when the membrane separates and reflows the cells, the unused n-alkanes are also returned to the fermentation process at the same time, Reduced the difficulty of separating n-alkanes in the dibasic acid extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] This example is a fermentation process without adding a fermentation and membrane separation coupling device. As a comparison of adding a fermentation and membrane separation coupling device, the process of fermenting and synthesizing long-chain dibasic acids is as follows:

[0019] The first step, culture medium preparation

[0020] ① Incline medium: malt juice agar medium;

[0021] ②Seed medium: glucose 20g / L, disodium hydrogen phosphate 2g / L, yeast extract 1g / L, corn steep liquor 2g / L, urea 0.5g / L, n-dodecane 100mL / L;

[0022] ③Fermentation medium: glucose 20g / L, disodium hydrogen phosphate 2g / L, yeast extract 1g / L, corn steep liquor 2g / L, urea 0.5g / L, NaCl 1g / L, KCl 1g / L, n-dodecane 300mL / L L, Tween 801g / L.

[0023] The second step, the activation of bacteria

[0024] A piece of Candida viswanathii was spread on a solid slant medium in a large test tube of 20×180 mm, and cultured at 27°C for 72 hours.

[0025] The third step, seed cultivation

[0026] The activ...

Embodiment 2

[0030] Follow the steps below to ferment and synthesize long-chain dibasic acids:

[0031] The first step, culture medium preparation

[0032] Same as Example 1

[0033] The second step, the activation of bacteria

[0034] Same as Example 1

[0035] The third step, seed cultivation

[0036] Same as Example 1

[0037] The fourth step, inoculation and fermentation

[0038] The culture obtained in the third step was inoculated into a 7.5L fermenter with 3L fermentation medium at an inoculum size of 10%, the initial pH was 6.0, the temperature was 27°C, and the ventilation rate was 1.0vvm, and cultured for 42h. Start the membrane module to make the bacteria return to the fermenter through the membrane module, and the permeate through the membrane module enters the extraction process. DC in fermenter 12When the concentration is less than 10g / L, reduce the outflow rate of the permeate through the separation coupling device; when the DC in the fermenter 12 When >50g / L, increa...

Embodiment 3

[0040] Follow the steps below to ferment and synthesize long-chain dibasic acids:

[0041] The first step, culture medium preparation

[0042] Same as Example 1

[0043] The second step, the activation of bacteria

[0044] Same as Example 1

[0045] The third step, seed cultivation

[0046] Same as Example 1

[0047] The fourth step, inoculation and fermentation

[0048] The culture obtained in the third step was inoculated into a 7.5L fermenter with 3L fermentation medium at an inoculum size of 10%, the initial pH was 6.0, the temperature was 27°C, and the ventilation rate was 1.0vvm, and cultured for 42h. Start the membrane module to make the cells return to the fermenter through the membrane module, and the permeate through the membrane module enters the extraction process. DC in fermenter 12 When the concentration is less than 10g / L, reduce the outflow rate of the permeate through the separation coupling device; when the DC in the fermenter 12 When >50g / L, increase...

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Abstract

The invention discloses a method for fermentation-membrane separation combined production of long-chain dicarboxylic acid and especially relates to a method for high yield production of dodecanedioic acid (DC12). The method comprises introducing a Candida viswanathii culture seed liquid into a liquid fermentation medium mixed solution which contains 5-40% (v / v) of C10-C18 n-alkane and 95-60% (v / v) of saccharic multielement substrate as a growth carbon source and has pH of 5.0-8.5, carrying out culture on the mixed solution under conditions of a temperature of 24-40 DEG C and ventilatory capacity of 0.1-3.0vvm for 42-194h, starting a fermentation-membrane separation combined device, returning the cells separated by the combined device into a fermentation cylinder, feeding the permeate liquid separated by the combined device into an extraction unit, preparing long-chain dicarboxylic acid, simultaneously, supplying a medium into the fermentation cylinder and carrying out fermentation. In n-dodecane transformation production of DC12, the method has an acid yield of 250g / L and an acid production rate of more than 1.6g / h.L, improves microbial density in fermentation, shortens a fermentation period, improves dicarboxylic acid production intensity and returns the unused n-dodecane into the fermentation unit.

Description

technical field [0001] The technical solution of the invention belongs to the technical field of fermentation engineering, and specifically relates to a method for coupling fermentation and membrane separation to produce long-chain dibasic acids. Background technique [0002] Long-chain dibasic acids are important raw materials for the synthesis of fragrances, engineering plastics, hot-melt adhesives, coatings, lubricating oils, resins and medicines. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment n-alkanes to produce long-chain dibasic acids. The ω-oxidation ability of domestic and foreign production strains is strong, and the acid production can reach 200g / L. However, a primary problem in the process of producing long-chain dibasic acids is low production efficiency. Therefore, in order to solve this problem, researchers have done a lot of work on str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C07C51/47C07C55/02C12R1/72
CPCC07C51/47C12P7/6409C07C55/21C07C55/02
Inventor 万印华曹伟锋杭晓风陈向荣
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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