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Lactobacillus plantarum used for silage alfalfa and use method thereof

A technology of Lactobacillus plantarum and alfalfa, applied in the field of lactic acid bacteria for silage, can solve the problems of high buffer energy, high moisture content of alfalfa, low sugar content, etc.

Active Publication Date: 2013-01-09
BEIJING SCITOP BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, alfalfa has high moisture content, relatively low sugar content, and high buffer energy, so it is not easy to silage under normal conditions.

Method used

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  • Lactobacillus plantarum used for silage alfalfa and use method thereof
  • Lactobacillus plantarum used for silage alfalfa and use method thereof
  • Lactobacillus plantarum used for silage alfalfa and use method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A strain of Lactobacillus plantarum, said Lactobacillus plantarum (L.plantarum Ps-9) is a lactic acid bacterium with good acid resistance, strong growth ability and fast acid production speed screened out from 100 strains of Lactobacillus plantarum; It has been preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee, and the preservation number is CGMCC No.5489.

[0020] Application of the plantaractobacillus in alfalfa silage.

[0021] For the activation and cultivation of Lactobacillus plantarum, inoculate the cryopreserved L. plantarum Ps-9 in the MRS liquid medium, culture it at 37°C for 18-22 hours, and subculture it for 2-3 times to obtain the activated L. plantarum Ps-9 strain; the composition of the MRS liquid medium is as follows: 10g peptone, 5g beef extract, 4g yeast extract powder, 20g glucose, 2g dipotassium hydrogen phosphate, 5g sodium acetate, 2g trisodium citrate, 1mL spit Temperatu...

Embodiment 2

[0035] The method for using Lactobacillus plantarum in alfalfa silage comprises the following steps:

[0036] The alfalfa raw material is naturally dried to a moisture content of 45%-55% for semi-dry silage. Take 1×10 5 The inoculation amount of cfu / g was inoculated into alfalfa raw materials, and the same volume of sterilized distilled water was added as a control group. After being vacuum-packed by a vacuum packaging machine, it was placed in a storage room for fermentation. After 30 days of fermentation, that is, after the fermentation is mature, a part of the silage is taken out for microbial composition and fermentation quality analysis.

[0037]Microbial composition analysis included counts of lactic acid bacteria, yeast, bacillus, mold, common bacteria and E. coli. Weigh 10g of fermented mature silage, add 90ml of sterilized water, shake fully, and then use sterilized water to dilute the sample by ten-fold dilution method, and select 100μL of appropriate diluent to ap...

Embodiment 3

[0047] The method for using Lactobacillus plantarum in alfalfa silage comprises the following steps:

[0048] Dry the raw material of alfalfa naturally until the water content is 45%-55% for semi-dry silage, add 3.0% and 5.0% molasses respectively, and mix the strain Ps-9 with 1×10 5 The inoculation amount of cfu / g was inoculated into alfalfa, and the same volume of sterilized distilled water was added as a control group. After being vacuum-packed by a vacuum packaging machine, it was placed in a storage room for fermentation. After 30 days of fermentation, that is, after the fermentation is mature, part of the silage is taken out for microbial composition and fermentation quality analysis.

[0049] Table 5 Effect of adding molasses on microbial composition of alfalfa semi-dry silage

[0050]

[0051] Note: different letters in the same column indicate significant difference (P<0.05); ND: not detected

[0052] From the analysis of microbial composition in Table 5, it can ...

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Abstract

The invention discloses a lactobacillus plantarum used for the silage alfalfa, which can improve the quality of the alfalfa silage feed. The lactobacillus plantarum used for the silage alfalfa has the technical scheme that the lactobacillus plantarum (L. plantarum Ps-9) is lactic acid bacteria which is screened from 100 plants of lactobacillus plantarum and has the advantages of favorable acid resistance, strong growth capability and high acid production speed. The bacterial strain of the bacteria is collected in the China general microbiological culture collection center with the collection number of CGMCC (China general microbiological culture collection center) No. 5489, and the collection data is 23rd, Nov., 2011. The invention also discloses a use method for the lactobacillus plantarum.

Description

technical field [0001] The invention relates to a strain of lactic acid bacteria for silage, in particular to a strain of Lactobacillus plantarum used for silage alfalfa capable of improving the quality of alfalfa silage and a use method thereof. Background technique [0002] Alfalfa (Medicago sativa) is a high-quality perennial legume grass with high yield, which can be cut several times a year, and is rich in nutrients such as protein, vitamins and minerals. For animal husbandry, alfalfa has good palatability, high rumen degradation rate, and high crude protein content, so alfalfa is an indispensable high-quality forage resource in animal husbandry production. However, for a long time, the preservation of alfalfa has been mainly sun-dried. The biggest disadvantage of this method is the serious loss of nutrients. Silage has become an indispensable basic feed for ruminants due to its fresh and juicy, high nutritional value, good palatability and long-term storage characteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/03C12R1/25
Inventor 于洁高鹏飞
Owner BEIJING SCITOP BIO TECH CO LTD
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